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AlphaLISA High Performance Human TNFα Detection Kit, 5,000 Assay Points

The AlphaLISA® High Performance (HP) Human TNFα Detection Kit is designed for the quantitative determination of human TNFα in serum, buffered solution, or cell culture medium using a homogeneous (no wash steps, no separation steps) assay.

This kit is the new and improved version of part number AL208.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Part Number: AL3157HV
Unit Size: 100 Assay Points
Part Number: AL3157C
Unit Size: 500 Assay Points
Part Number: AL3157F
Unit Size: 5,000 Assay Points
  • Tumor necrosis factor alpha (TNFα) is a multifunctional proinflammatory cytokine synthesized mainly by nucleated blood cells as a 233 aa type II transmembrane protein which is cleaved by ADAM17 between aa 76-77 to form a soluble homotrimeric complex. TNFα plays a role in lipid metabolism, coagulation, and endothelial function and has been associated with cancer, infection and inflammation (including inflammatory bowel disease), ischemia/reperfusion injury and heart failure, and insulin resistance.

    Formats
    • Our 100 assay point kit allows you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
    • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
    • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
    Features
    • No-wash steps, no separation steps
    • ELISA alternative technology
    • Sensitive detection
    • Broad sample compatibility
    • Small sample volume
    • Results in less than 3 hours
    • Half the time of an ELISA assay

    AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

  • Application
    Protein Analysis & Detection
    Protein Detection
    Assay Points
    5000
    Assay Target Class
    Cytokine
    Assay Technology
    Alpha
    Automation Compatible
    Yes
    Brand
    AlphaLISA
    Detection Method
    Alpha
    Experimental Type
    In vitro
    Target Species
    Human
    Therapeutic Area
    Autoimmunity
    Cardiovascular
    Immuno-oncology
    Inflammation
    Neuroscience
    Oncology
    Unit Size
    5,000 Assay Points

Resources

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Application Note
Homogeneous detection of cytokines from PBMC supernatants using AlphaLISA High Performance assays

Cytokines play an essential role in inflammatory processes, leading to their involvement in viral and bacterial infections, as...

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