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AlphaLISA High Performance Human IL-8 Detection Kit, 100 Assay Points

The AlphaLISA® High Performance (HP) Human IL-8 Detection Kit is designed for the quantitative determination of human IL-8 in serum, buffered solution, or cell culture medium using a homogeneous (no wash steps, no separation steps) assay.

This kit is the new and improved version of part number AL224.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Product Variants
Part number: AL3164HV
Unit Size: 100 Assay Points
List price: USD 896.90
Your price:
USD 896.90
USD 896.90 /each
Part number: AL3164C
Unit Size: 500 Assay Points
List price: USD 2,261.00
Your price:
USD 0.00
USD 2,261.00 /each
Part number: AL3164F
Unit Size: 5,000 Assay Points
List price: USD 14,900.00
Your price:
USD 0.00
USD 14,900.00 /each

Overview

Interleukin 8 (IL-8 or CXCL8), a member of the ELR+ CXC chemokine family, is an 8.4 kDa polypeptide that forms homodimers in vivo. IL-8 is secreted by several types of cells, including fibroblasts, monocytes, macrophages, and endothelial cells, in response to inflammatory stimuli. It is a chemoattractant and activator for neutrophils, directing them from peripheral blood to the site of inflammation. It is also a potent angiogenic factor promoting endothelial and epithelial migration and proliferation in several cancers and is associated with metastasis. It signals through two specific G protein-coupled receptors, CXCR1 and CXCR2, sharing ~77% identity.

Formats
  • Our 100 assay point kit allows you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
Features
  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Small sample volume
  • Results in less than 3 hours
  • Half the time of an ELISA assay

AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

Specifications

Application
Protein Analysis & Detection
Protein Detection
Assay Points
100
Assay Target Class
Cytokine
Assay Technology
Alpha
Automation Compatible
Yes
Brand
AlphaLISA
Detection Method
Alpha
Experimental Type
In vitro
Shipping Conditions
Shipped in Blue Ice
Target Species
Human
Therapeutic Area
Autoimmunity
Immuno-oncology
Neuroscience
Oncology
Unit Size
100 Assay Points

Video gallery

Resources

1-3 of 3 Resources
Flyer
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Application Note Icon Image
Application Note
Homogeneous detection of cytokines from PBMC supernatants using AlphaLISA High Performance assays

Cytokines play an essential role in inflammatory processes, leading to their involvement in viral and bacterial infections, as...

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SDS, COAs, Manuals and more

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