AlphaLISA Human Oncostatin M Detection Kit, 100 Assay Points

Formats:

• Our HV (100 assay point) kits allow you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
• Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
• Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features:

• No-wash steps, no separation steps
• ELISA alternative technology
• Sensitive detection
• Broad sample compatibility
• Small sample volume
• Results in less than 3 hours
• Half the time of an ELISA assay

Oncostatin M, also known as OSM, is a growth factor encoded by the OSM gene in humans. OSM is a pleiotropic cytokine that belongs to the interleukin 6 group of cytokines (also include granulocyte colony-stimulating factor), most closely resembles leukemia inhibitory factor (LIF) in both structure and function. The fully active 196 residue OSM is the predominant form isolated from activated monocytes and T lymphocytes, and corresponds to a glycoprotein of 28 Kda. It is proving important in liver development, haematopoeisis, inflammation and possibly CNS development. It is also associated with bone formation and destruction. It is a growth regulator which inhibits the proliferation of a number of tumor cell lines and stimulates normal fibroblasts, AIDS-Kaposi’s sarcoma. It regulates cytokine production, including IL-6, G-CSF (granulocyte colony-stimulating factor) and GM-CSF from endothelial cells. OSM signals through cell surface receptors that contain the protein gp130. The type I receptor is composed of gp130 and LIFR, the type II receptor is composed of gp130 and OSMR.

AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.