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AlphaLISA Human MMP-12 Detection Kit, 100 Assay Points

The AlphaLISA® immunoassay kit for human MMP-12 enables the detection and quantitation of human matrix metalloproteinase-12 in serum or cell culture medium using a homogeneous AlphaLISA assay (no wash steps). The kit was designed to detect both MMP12 and pro-MMP12.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Product Variants
Part number: AL375HV
Unit Size: 100 Assay Points
List price: USD 700.73
Your price:
USD 700.73
USD 700.73 /each
Part number: AL375C
Unit Size: 500 assay points
List price: USD 2,323.00
Your price:
USD 0.00
USD 2,323.00 /each
Part number: AL375F
Unit Size: 5,000 Assay Points
List price: USD 15,320.00
Your price:
USD 0.00
USD 15,320.00 /each

Overview

Formats:

  • Our 100 assay point kit allows you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features:

  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Small sample volume
  • Results in less than 3 hours
  • Half the time of an ELISA assay

Matrix metalloproteinase 12 (MMP12, macrophage elastase ), a member of the matrix metalloproteinase family, is an enzyme composed of a pro domain, a catalytic domain containing the zinc-binding site, and a C-terminal hemopexin-like domain. MMP12 can degrade soluble and insoluble elastin and type IV collagen, fibronectin, laminin, vitronectin, proteoglycans, chondroitin sulfate, myelin basic protein, alpha 1-antitrypsin, and plasminogen. Like other MMPs, MMP12 is involved in the degradation of basement membranes and thus cancer development. This kit has been designed for the detection of Human MMP12 in serum and cell culture supernatants.

AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

Specifications

Application
Protein Analysis & Detection
Assay Points
100
Assay Target Class
Biomarker
Protein
Automation Compatible
Yes
Brand
AlphaLISA
Detection Method
Alpha
Experimental Type
In vitro
Format
Microplates
Shipping Conditions
Shipped in Blue Ice
Therapeutic Area
Oncology
Unit Size
100 Assay Points

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