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AlphaLISA Human IL1-RA Detection Kit, 100 Assay Points

The AlphaLISA® Human IL-1RA Detection Kit is designed for detection and quantitation of human interleukin-1RA in cell culture media or serum using a homogeneous (no-wash steps, no separation steps) assay.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).

Product Variants
Part number: AL358HV
Unit Size: 100 Assay Points
List price: USD 700.73
Your price:
USD 700.73
USD 700.73 /each
Part number: AL358C
Unit Size: 500 assay points
List price: USD 2,322.00
Your price:
USD 0.00
USD 2,322.00 /each
Part number: AL358F
Unit Size: 5,000 Assay Points
List price: USD 15,320.00
Your price:
USD 0.00
USD 15,320.00 /each

Overview

Formats:

  • Our 100 assay point kit allows you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features:

  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Small sample volume
  • Results in less than 3 hours
  • Half the time of an ELISA assay

Interleukin-1 Receptor Antagonist (IL1RA) is a member of the interleukin-1 cytokine family. Immune, epithelial, and adipose cells secrete IL1RA which will bind to the interleukin-1 receptor and inhibit pro-inflammatory signaling through IL-1α and IL-1β. Studies have shown that IL1RA plays a key role in suppressing the pro-inflammatory effects of IL-1 in diseases like rheumatoid arthritis. Additionally, it has been observed that IL1RA may play an important role in diseases like osteoporosis where IL1RA levels are suppressed compared to controls. Furthermore, it has been observed that IL-1 cytokines are abundantly present at tumor sites and the addition of IL1RA can suppress growth of IL-1 producing tumors.

AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

Specifications

Application
Protein Analysis & Detection
Assay Points
100
Assay Target Class
Cytokine
Automation Compatible
Yes
Brand
AlphaLISA
Detection Method
Alpha
Experimental Type
In vitro
Format
Microplates
One Unit Contains
100.0 assay points
Shipping Conditions
Shipped in Blue Ice
Therapeutic Area
Inflammation
Unit Size
100 Assay Points

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