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AlphaLISA Human IL-17F Detection Kit, 100 Assay Points

The AlphaLISA® Human IL-17F Detection Kit is designed for detection and quantitation of human interleukin 17F in cell culture media or serum using a homogeneous (no-wash steps, no separation steps) assay.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Part Number: AL391HV
Unit Size: 100 Assay Points
Part Number: AL391C
Unit Size: 500 assay points
Part Number: AL391F
Unit Size: 5,000 Assay Points
  • Formats:

    • Our 100 assay point kit allows you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
    • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
    • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

    Features:

    • No-wash steps, no separation steps
    • ELISA alternative technology
    • Sensitive detection
    • Broad sample compatibility
    • Small sample volume
    • Results in less than 3 hours
    • Half the time of an ELISA assay

    IL17F, also known as human interleukin 17, is a pro-inflammatory cytokine produced by T-helper cells. This cytokine has been found to inhibit the angiogenesis and endothelial cells and induce endothelial cells to produce other cytokines. Levels of this cytokine play an important role in asthma and chronic inflammatory disease such as psoriasis, multiple sclerosis, and rheumatoid arthritis. The present kit is designed to detect IL17F in serum, plasma, cell culture supernatants, and cell lysates.

    AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

  • Application
    Protein Analysis & Detection
    Assay Points
    100
    Assay Target Class
    Cytokine
    Automation Compatible
    Yes
    Brand
    AlphaLISA
    Detection Method
    Alpha
    Experimental Type
    In vitro
    Quantity in a Package Amount
    1.0 Units
    Species
    Human
    Shipping Conditions
    Shipped in Blue Ice
    Therapeutic Area
    Inflammation
    Unit Size
    100 Assay Points

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