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AlphaLISA Human Heme oxygenase 1 Detection Kit, 5,000 Assay Points

The AlphaLISA® immunoassay kit for human heme oxygenase 1 (HO-1) enables the quantitative determination of human HO-1 in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Part Number: AL3026HV
Unit Size: 100 Assay Points
Part Number: AL3026C
Unit Size: 500 Assay Points
Part Number: AL3026F
Unit Size: 5,000 Assay Points
  • Formats:

    • Our 100 assay point kit allows you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
    • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
    • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

    Features:

    • No-wash steps, no separation steps
    • ELISA alternative technology
    • Sensitive detection
    • Broad sample compatibility
    • Small sample volume
    • Results in less than 3 hours
    • Half the time of an ELISA assay

    AlphaLISA technology allows the detection of molecules of interest in buffer, cell culture media, serum and plasma in a highly sensitive, quantitative, reproducible and user-friendly mode. In an AlphaLISA assay, a Biotinylated Anti-Analyte Antibody binds to the Streptavidin-coated Alpha Donor beads, while another Anti-Analyte Antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads provokes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

    Heme oxygenases are enzymes that are involved in the degradation of heme. They cleave the heme ring to create billiverdin, which then becomes bilirubin due to the action of bilirubin reductase. This cascade allows for the removal of heme in damaged red blood cells, as well as heme bound irreversibly to molecules such as carbon monoxide. Heme oxygenases have been characterized as markers for oxidative stress, metabolic disorders and inflammation. Heme oxygenase 1 is induced in the bloodstream by oxidative stress. Its role appears to be protective. Production of carbon monoxide from the cleavage heme ring plays a role by regulating nitric oxide synthase, thus regulating blood pressure and activating parts of the immune system.

  • Application
    Protein Analysis & Detection
    Assay Points
    5000
    Assay Target Class
    Protein
    Automation Compatible
    Yes
    Brand
    AlphaLISA
    Detection Method
    Alpha
    Experimental Type
    In vitro
    Format
    Microplates
    Therapeutic Area
    Inflammation
    Unit Size
    5,000 Assay Points