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AlphaLISA Glutathione Acceptor Beads, 25 mg

AlphaLISA® Acceptor beads conjugated to glutathione. These beads can be used to capture GST-tagged proteins and peptides.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Part Number: AL109R
Unit Size: 25 mg
Part Number: AL109C
Unit Size: 250 µg
Part Number: AL109M
Unit Size: 5 mg
  • These beads can be used to capture GST-tagged proteins and peptides, and can be used in conjunction with Alpha Donor beads to create AlphaLISA® no-wash assays for:

    • Protein-protein interaction assays
    • Protein detection assays
    • Enzymatic assays

    In a typical AlphaLISA assay, 1 mg of Acceptor beads is sufficient to run 1,000-2,000 wells using a 50 µL reaction volume.

    Features:

    • No-wash steps, no separation steps
    • Ease-of-use: few addition steps, fast assay development
    • Broad range of affinities: detect strong or weak interactions, from pM to mM affinity
    • Distance: measure very large protein or antibody complexes – spanning up to 200 nm or more
    • High avidity: multiple binding sites on each bead enables use of nanomolar concentrations of antibodies or proteins, as well as use of low affinity binders

    AlphaScreen® and AlphaLISA® are bead-based assay technologies used to study biomolecular interactions in a microplate format. The acronym ""Alpha"" stands for amplified luminescent proximity homogeneous assay. As the name implies, some of the key features of these technologies are that they are non-radioactive, homogeneous proximity assays. Binding of molecules captured on the beads leads to an energy transfer from one bead to the other, ultimately producing a luminescent/fluorescent signal. To understand how a signal is produced, one must begin with an understanding of the beads. AlphaScreen and AlphaLISA assays require two bead types: Donor beads and Acceptor beads. Each bead type contains a different proprietary mixture of chemicals, which are key elements of the AlphaScreen technology. Donor beads contain a photosensitizer, phthalocyanine, which converts ambient oxygen to an excited and reactive form of O2, singlet oxygen, upon illumination at 680 nm. Please note that singlet oxygen is not a radical; it is molecular oxygen with a single excited electron. Like other excited molecules, singlet oxygen has a limited lifetime prior to falling back to ground state. Within its 4 µsec half-life, singlet oxygen can diffuse approximately 200 nm in solution. If an Acceptor bead is within that proximity, energy is transferred from the singlet oxygen to thioxene derivatives within the Acceptor bead, subsequently culminating in light production at 520-620 nm (AlphaScreen) or at 615 nm (AlphaLISA). In the absence of an Acceptor bead, singlet oxygen falls to ground state and no signal is produced. This proximity-dependent chemical energy transfer is the basis for AlphaScreen's homogeneous nature.

  • Antibody Conjugates
    Glutathione
    Application
    Protein-protein interactions
    Automation Compatible
    Yes
    Bead Type or Core Bead Type
    AlphaLISA Acceptor
    Brand
    AlphaLISA
    Detection Method
    Alpha
    Experimental Type
    In vitro
    Format
    Microplates
    Species
    Human
    Unit Size
    25 mg

Resources

1-6 of 6 Resources
Application Note Icon Image
Application Note
A Fast and Simple Chemiluminescent Assay for Monitoring of DNA-Protein Interactions
DNA-protein, DNA:protein, binding, interaction, protein:DNA, protein-DNA, AlphaLISA, EMSA
Guide book
Alpha Protein-Protein Interaction Quick Start Guide
AlphaLISA, protein, assay, immunoassay
Guide book
Alpha Protein-Protein Interaction Quick Start Guide
AlphaLISA, protein, assay, immunoassay
Application Note Icon Image
Application Note
Biochemical binding ADCC assays utilizing AlphaLISA toolbox reagents for the characterization of hIgGs and FcγR1A.
AlphaLISA, toolbox, ADCC, biochemical, hIgG, assays
Guide book
ELISA to AlphaLISA Immunoassay Conversion Guide
ELISA conversion, AlphaLISA, long guide
Guide book
ELISA to AlphaLISA Immunoassay Conversion Guide
ELISA conversion, AlphaLISA, long guide