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AlphaLISA Human GM-CSF Detection Kit, 500 Assay Points

The AlphaLISA® Human Granulocyte/Macrophage Colony-Stimulating Factor (GM-CSF) Detection Kit is designed for detection and quantitation of human GM-CSF in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).

Product Variants
Part number: AL216C
Unit Size: 500 Assay Points
List price: USD 2,392.00
Your price:
USD 2,392.00
USD 2,392.00 /each
Part number: AL216F
Unit Size: 5,000 Assay Points
List price: USD 15,950.00
Your price:
USD 0.00
USD 15,950.00 /each

Overview

Formats:

  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features:

  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Small sample volume
  • Results in less than 3 hours
  • Half the time of an ELISA assay

Colony-stimulating factors (CSFs) are proteins necessary for the survival, proliferation, and differentiation of hematopoietic progenitor cells. The human Granulocyte/Macrophage colony-stimulating factor (GM-CSF) is a ~23 kDa glycosylated protein (144-aa), encoded by the CSF2 gene. It has been originally purified from culture media conditioned by lung tissue from endotoxin injected mice, having the capacity to stimulate the formation of both granulocyte and macrophage colonies.

AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

Specifications

Application
Protein Analysis & Detection
Assay Points
500
Assay Target Class
Cytokine
Automation Compatible
Yes
Brand
AlphaLISA
Detection Method
Alpha
Experimental Type
In vitro
Format
Microplates
Species
Mouse
Shipping Conditions
Shipped in Blue Ice
Therapeutic Area
Inflammation
Unit Size
500 Assay Points

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SDS, COAs, Manuals and more

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