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AlphaLISA Human Fibronectin Detection Kit, 100 Assay Points

The AlphaLISA® immunoassay kit for human fibronectin enables the quantitative determination of human fibronectin in serum, buffered solution, and cell culture media using a homogeneous AlphaLISA assay (no wash steps).

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Part Number: AL3056HV
Unit Size: 100 Assay Points
Part Number: AL3056C
Unit Size: 500 Assay Points
Part Number: AL3056F
Unit Size: 5,000 Assay Points
  • Formats:

    • Our 100 assay point kit allows you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
    • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
    • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

    Features:

    • No-wash steps, no separation steps
    • ELISA alternative technology
    • Sensitive detection
    • Broad sample compatibility
    • Small sample volume
    • Results in less than 3 hours
    • Half the time of an ELISA assay

    AlphaLISA technology allows the detection of molecules of interest in buffer, cell culture media, serum and plasma in a highly sensitive, quantitative, reproducible and user-friendly mode. In an AlphaLISA assay, a Biotinylated Anti-Analyte Antibody binds to the Streptavidin-coated Alpha Donor beads, while another Anti-Analyte Antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads provokes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

    Fibronectin is a large glycoprotein of the extracellular matrix that exists in two forms: insoluble (cellular) and as a soluble plasma protein. The primary functions of fibronectin are its involvement in cell adhesion, growth, migration, and differentiation. Fibronectin is known to contribute to the wound healing process where it will accumulate at the site of injury to help form blood clots and aid in the development of new tissues. Fibronectin levels in serum and plasma have been identified to increase significantly after major traumas. Further, Fibronectin levels have been observed to be significantly altered in disease states of certain types of cancer and also diabetes mellitus. This AlphaLISA kit has been designed to detect and quantify Human Fibronectin in human serum and cell culture supernatants.

  • Application
    Protein Analysis & Detection
    Assay Points
    100
    Assay Target Class
    Biomarker
    Protein
    Automation Compatible
    Yes
    Brand
    AlphaLISA
    Detection Method
    Alpha
    Therapeutic Area
    Oncology
    Unit Size
    100 Assay Points