AlphaLISA Human ErbB2 (HER2) Detection Kit, 500 Assay Points

The antibodies target the extracellular domain of the HER2 receptor. The analyte in this kit consists of the extracellular domain of human HER2 fused to the Fc region of human IgG1.

Formats:

• Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
• Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features:

• No-wash steps, no separation steps
• ELISA alternative technology
• Sensitive detection
• Broad sample compatibility
• Small sample volume
• Results in less than 3 hours
• Half the time of an ELISA assay

Human epidermal growth factor receptor 2 (ERBB2, NEU, or HER2) is a type I membrane glycoprotein that is a member of the ERBB family of tyrosine kinase receptors. It serves as a receptor for the epidermal growth factor (EGF) family of growth factors. HER2 is widely expressed in epithelial cells and has also been found to be over-expressed in a large number of breast carcinomas. It is an orphan receptor with no known ligand, as it acts as a heterodimer with other members of the EGF receptors family. HER2 appears to play roles in development, cancer, communication at the neuromuscular junction, and regulation of cell growth and differentiation. HER2 can be shed from the cell surface as a soluble protein (sHER2) via proteolytic cleavage by an unidentified protease. sHER2 seems to be an important serum biomarker in certain types of cancer.

AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.