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AlphaLISA Human EPO Detection Kit, 5,000 Assay Points

The AlphaLISA® Human Erythropoietin (EPO) Detection Kit is designed for detection and quantitation of human EPO in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Part Number: AL206C
Unit Size:
Part Number: AL206F
Unit Size:
  • Formats:

    • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
    • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

    Features:

    • No-wash steps, no separation steps
    • ELISA alternative technology
    • Sensitive detection
    • Broad sample compatibility
    • Small sample volume
    • Results in less than 3 hours
    • Half the time of an ELISA assay

    Human Erythropoietin hormone (EPO) is a 34 kDa glycoprotein produced by kidney cells which are sensitive to oxygen levels in the blood and release EPO upon decreased of oxygen levels in the blood. EPO in the bone marrow then stimulates differentiation and development of red blood cells and initiates the production of haemoglobin thereby increasing the oxygen-carrying capacity of the blood. Polycythemia, a disorder in which there is an excess of red blood cells has been associated with elevated levels of EPO whereas lower than normal levels of EPO is used in chronic renal failure detection.

    AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

  • Application
    Protein Analysis & Detection
    Assay Points
    5000
    Assay Target Class
    Cytokine
    Automation Compatible
    Yes
    Brand
    AlphaLISA
    Detection Method
    Alpha
    Experimental Type
    In vitro
    Format
    Microplates
    Species
    Human
    Therapeutic Area
    Central Nervous System