AlphaLISA Human CXCL9 Detection Kit, 500 Assay Points

Formats:

• Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
• Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features:

• No-wash steps, no separation steps
• ELISA alternative technology
• Sensitive detection
• Broad sample compatibility
• Small sample volume
• Results in less than 3 hours
• Half the time of an ELISA assay

C-X-C Motif Chemokine 9 (CXCL9), previously called MIG, is a 14 kDa protein belonging to the intercrine alpha (chemokine CXC) family. Its induction is enhanced by TNFa in dermal fibroblasts and vein endothelial cells. The synthesis of CXCL9 is specifically induced in macrophages, monocytes, neutrophils, APC, B cells, and eosinophils by IFN? and mediated via the JAK-STAT signaling pathway. The main function of this chemokine is the recruitment of leukocytes to sites of infection and inflammation. Some studies have shown that CXCL9 is active against Gram-negative and Gram-positive bacteria. CXCL9 may play a role as a mediator of T-cell recruitment and activation in some diseases like psoriasis and pulmonary disease. CXCL9 is expressed in allogeneic skin grafts several days before completion of rejection.

AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.