The AlphaLISA™ Human CXCL1/GROα Detection Kit is designed for detection and quantitation of human CXCL1/GROα in cell culture media or serum using a homogeneous (no-wash steps, no separation steps) assay.
Feature | Specification |
---|---|
Application | Protein Quantification |
Dynamic Range | 0.5 - 30,000 pg/mL |
Limit of Detection | 0.5 pg/mL |
Limit of Quantification | 1.8 pg/mL |
Sample Volume | 5 µL |
The AlphaLISA™ Human CXCL1/GROα Detection Kit is designed for detection and quantitation of human CXCL1/GROα in cell culture media or serum using a homogeneous (no-wash steps, no separation steps) assay.
Formats:
Features:
C-X-C Motif Chemokine 1 (CXCL1) is an 11 kDa chemokine and is part of the intercrine alpha (chemokine CXC) family. The secreted protein is proteolytically processed at the N-terminus and the processed form is usually referred to as GRO-α. CXCL1 is expressed by macrophages, neutrophils and epithelial cells. Its synthesis is induced by a variety of inflammatory mediators such as PDGF, M-CSF, TNFα, LPS, and TLR 4. The major role of CXCL1 is its chemoacttractant activity on neutrophils. CXCL1 plays a role in inflammation and exerts its effects on endothelial cells in an autocrine way. CXCL1 is implicated in spinal cord development, angiogenesis, inflammation, wound healing, and tumorigenesis. CXCL1 is overexpressed in invasive bladder cancer and is secreted by human melanoma cells.
AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
Application |
Protein Quantification
|
---|---|
Automation Compatible |
Yes
|
Brand |
AlphaLISA
|
Detection Modality |
Alpha
|
Dynamic Range |
0.5 - 30,000 pg/mL
|
Limit of Detection |
0.5 pg/mL
|
Limit of Quantification |
1.8 pg/mL
|
Product Group |
Kit
|
Sample Volume |
5 µL
|
Shipping Conditions |
Shipped in Blue Ice
|
Target |
CXCL1/GROα
|
Target Class |
Cytokines
|
Target Species |
Human
|
Technology |
Alpha
|
Therapeutic Area |
Inflammation
|
Unit Size |
500 assay points
|
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Cytokines play a vital role in both innate and adaptive immunity and are known for their ability to exert diverse functions on...
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