Skip to main content

AlphaLISA Human CTLA4-CD80 Binding Kit, 5,000 Assay Points

The AlphaLISA® CTLA-4/CD80 binding kit is designed for the detection of binding activity between human CTLA-4 and CD80, using a fast and simple homogeneous AlphaLISA assay (no wash steps). This assay can be used to screen for small molecules that inhibit binding, as a competitive ligand binding (CLB) assay to screen therapeutic blocking antibodies, and for potency assays.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Part Number: AL3046F
Unit Size: 5,000 Assay Points
Part Number: AL3046C
Unit Size: 500 Assay Points
  • Features:

    • No-wash steps, no separation steps
    • ELISA alternative technology
    • Sensitive detection
    • Broad range of affinities
    • High avidity
    • Results in less than 3 hours
    • Half the time of an ELISA assay

    AlphaLISA technology allows the detection of molecules of interest in buffer, cell culture media, serum and plasma in a highly sensitive, quantitative, reproducible and user-friendly mode. In an AlphaLISA assay, a biotinylated CD80 binds to the Streptavidin-coated Alpha Donor beads, while His tagged CTLA-4 is captured by Anti-His AlphaLISA Acceptor beads. When CD80 binding to CTLA-4 happens, Donor beads and Acceptor beads come into close proximity. The excitation of the Donor beads provokes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

    Human Cytotoxic T-lymphocyte-associated protein 4 (CTLA-4), also known as CD152 (cluster of differentiation 152), is a cell membrane receptor and a member of immunoglobulin superfamily. CTLA-4 is expressed once a T cell becomes active and modulates T cell signals by blocking the CD80 (B7.1) and CD86 (B7.2) ligands from binding to CD28. CTLA-4, functioning as an immune checkpoint, downregulates T cell immune responses. Because of its profound inhibitory role blocking CTLA-4 and CD80 or CD86 binding has been considered as promising therapeutic target for human autoimmune disease and cancers.

  • Automation Compatible
    Yes
    Application
    Protein-protein interactions
    Assay Points
    5000
    Assay Target
    CTLA4,CD80
    Assay Target Class
    Protein
    Detection Method
    Alpha
    Therapeutic Area
    Immuno-oncology
    Brand
    AlphaLISA
    Unit Size
    5,000 Assay Points

Resources

1-1 of 1 Resources
Flyer
Reagent solutions for autoimmunity research.
AlphaLISA, Alpha, HTRF, SureFire, autoimmunity, flyer