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AlphaLISA Plant Cry2A Detection Kit, 500 Assay Points

The AlphaLISA™ Cry2A Detection Kit is designed for detection and quantitation of Cry2A in GMO seeds, leaves, and plant extracts in a homogeneous (no-wash steps, no separation steps) assay.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

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Feature Specification
Application Protein Quantification
Dynamic Range 12 - 300,000 pg/mL
Limit of Detection 12 pg/mL
Limit of Quantification 89 pg/mL
Sample Volume 5 µL

The AlphaLISA™ Cry2A Detection Kit is designed for detection and quantitation of Cry2A in GMO seeds, leaves, and plant extracts in a homogeneous (no-wash steps, no separation steps) assay.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

Click to copy promo code to clipboard.
SAVE 15% NOW on online orders. Use promo code below.
YEAREND15
Offer valid until 12/15. Terms and conditions apply.
Product Variants
Unit Size: 100 Assay Points
Part #:
AL403HV
List Price
USD 944.10
Unit Size: 500 Assay Points
Part #:
AL403C
List Price
USD 2,311.00
Unit Size: 5,000 Assay Points
Part #:
AL403F
List Price
USD 14,690.00

Overview

Formats:

  • Our HV (100 assay point) kits allow you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features:

  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Small sample volume
  • Results in less than 3 hours
  • Half the time of an ELISA assay

Cry2A is an insecticidal protein and is produced by the naturally occurring soil bacterium Bacillus thuringiensis subsp. kurstaki (Btk). The protein is used extensively in agricultural farming as a direct application pesticide. The gene encoding Cry2A has been widely introduced to genomes of many crops (e.g. corn, cotton, and soybean) to create genetically modified organisms (GMO). During growth, genetically modified crops produce the Cry2A protein that confers protection against certain insect pests.

AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

Specifications

Application
Protein Quantification
Automation Compatible
Yes
Brand
AlphaLISA
Detection Modality
Alpha
Dynamic Range
12 - 300,000 pg/mL
Limit of Detection
12 pg/mL
Limit of Quantification
89 pg/mL
Product Group
Kit
Sample Volume
5 µL
Shipping Conditions
Shipped in Blue Ice
Target
Cry2A
Target Class
Biomarkers
Target Species
Plant
Technology
Alpha
Unit Size
500 Assay Points

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Alpha technolgy enables the rapid and straightforward mesaure of virtually any target. This includes enzymes, receptor-ligand...

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