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AlphaLISA Human cIAP1 BIR2 Binding Kit, 500 Assay Points

The AlphaLISA® Human cIAP1 BIR2 Binding Kit is designed to screen and characterize compounds that specifically bind the E3 ligase, cIAP1 BIR2 domain, using Alpha Technology.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Product Variants
Part number: AL3166C
Unit Size: 500 Assay Points
List price: USD 3,338.00
Your price:
USD 3,338.00
USD 3,338.00 /each
Part number: AL3166F
Unit Size: 5,000 Assay Points
List price: USD 16,370.00
Your price:
USD 0.00
USD 16,370.00 /each

Overview

cIAP1, also called BIRC2, is a member of the Inhibitor of Apoptosis Proteins (IAP) family. IAP proteins are involved in multiple biological processes, such as innate immunity, and play an important role in apoptosis inhibition. cIAP1 overexpression has been associated with cancer resistance, making it an attractive target in cancer therapy where several therapeutic strategies have been investigated, such as SMAC mimetics.

Formats:
  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
Features:
  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Small sample volume

AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

Specifications

Application
Protein Detection
Assay Points
500
Assay Technology
Alpha
Automation Compatible
Yes
Brand
AlphaLISA
Detection Method
Alpha
Experimental Type
In vitro
Target Species
Human
Therapeutic Area
Autoimmunity
Neuroscience
Oncology
Unit Size
500 Assay Points

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