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AlphaLISA Human CD70 Detection Kit, 100 Assay Points

The AlphaLISA® immunoassay kit for human CD70 enables the quantitative determination of human CD70 in buffer, cell culture media, and cell lysates using a homogeneous AlphaLISA assay (no wash steps).

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Part Number: AL3103HV
Unit Size: 100 Assay Points
Part Number: AL3103C
Unit Size: 500 Assay Points
Part Number: AL3103F
Unit Size: 5,000 Assay Points
  • Formats:

    • Our 100 assay point kit allows you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
    • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
    • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

    AlphaLISA features:

    • No-wash steps, no separation steps
    • ELISA alternative technology
    • Sensitive detection
    • Broad sample compatibility
    • Small sample volume
    • Results in less than 3 hours
    • Half the time of an ELISA assay

    AlphaLISA technology allows the detection of molecules of interest in buffer, cell culture media, serum and plasma in a highly sensitive, quantitative, reproducible and user-friendly mode. In an AlphaLISA assay, a Biotinylated Anti-Analyte Antibody binds to the Streptavidin-coated Alpha Donor beads, while another Anti-Analyte Antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads provokes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

    Cluster of differentiation 70 (CD70), also known as CD27L or TNFSF7, is a type II transmembrane receptor protein expressed on activated T and B cells. It binds to CD27 on mature T-cells and a subset of thymocytes. Ligation of CD70 results in the proliferation of costimulated T cells. CD70 expression has been shown to be upregulated in range of lymphomas. Treatment with anti-CD70 antibodies has been introduced as a possible treatment for CD70+ tumors.

  • Assay Points
    100
    Assay Target Class
    Protein
    Automation Compatible
    Yes
    Brand
    AlphaLISA
    Detection Method
    Alpha
    Unit Size
    100 Assay Points

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