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AlphaLISA Human CD28 Detection Kit, 5,000 Assay Points

The AlphaLISA® immunoassay kit for human CD28 enables the quantitative determination of human CD28 in buffered solution and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Part Number: AL3044HV
Unit Size:
Part Number: AL3044C
Unit Size:
Part Number: AL3044F
Unit Size:
  • Formats:

    • Our 100 assay point kit allows you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
    • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
    • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

    Features:

    • No-wash steps, no separation steps
    • ELISA alternative technology
    • Sensitive detection
    • Broad sample compatibility
    • Small sample volume
    • Results in less than 3 hours
    • Half the time of an ELISA assay

    AlphaLISA technology allows the detection of molecules of interest in buffer, cell culture media, serum and plasma in a highly sensitive, quantitative, reproducible and user-friendly mode. In an AlphaLISA assay, a Biotinylated Anti-Analyte Antibody binds to the Streptavidin-coated Alpha Donor beads, while another Anti-Analyte Antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads provokes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

    CD28 (Cluster of Differentiation 28) is one of the proteins expressed on T cells that provide co-stimulatory signals required for T cell activation and survival. T cell stimulation through CD28 in addition to the T-cell receptor (TCR) can provide a potent signal for the production of various interleukins (IL-6 in particular). CD28 is the receptor for CD80 (B7.1) and CD86 (B7.2) proteins. When activated by Toll-like receptor ligands, the CD80 expression is upregulated in antigen presenting cells (APCs) and the CD86 expression on antigen presenting cells is constitutive. CD28 is involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival. In healthy subjects, soluble CD28 levels in plasma are about 0.8 ng/mL.

  • Application
    Protein Analysis & Detection
    Assay Points
    5000
    Assay Target Class
    Protein
    Automation Compatible
    Yes
    Brand
    AlphaLISA
    Detection Method
    Alpha
    Format
    Microplates
    Therapeutic Area
    Immuno-oncology

Resources

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Application Note Icon Image
Application Note
Protocol Optimization for Detection and Quantification of Membrane-Bound Proteins Using AlphaLISA Te
alphalisa, cell surface, membrane protein, detection
Flyer
Reagent solutions for autoimmunity research.
alphalisa, alpha, htrf, surefire, autoimmunity, flyer