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AlphaLISA Bovine and Ovine IL-10 Detection Kit, 100 Assay Points

The AlphaLISA™ immunoassay kit for bovine IL-10 enables the detection and quantitation of bovine IL-10 (bIL10) in serum, buffered solution and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps). This kit also detects Ovine IL-10.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).

Product Variants
Part number: AL546HV
Unit Size: 100 Assay Points
List price: USD 1,094.00
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USD 1,094.00
USD 1,094.00 /each
Part number: AL546C
Unit Size: 500 assay points
List price: USD 2,991.00
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USD 0.00
USD 2,991.00 /each
Part number: AL546F
Unit Size: 5,000 Assay Points
List price: USD 19,730.00
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USD 0.00
USD 19,730.00 /each

Overview

Formats:

  • Our 100 assay point kit allows you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features:

  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Small sample volume
  • Results in less than 3 hours
  • Half the time of an ELISA assay

Bovine Interleukin 10 (bIL10) is a member of the IL10 family of cytokines (IL10, IL-19, IL-20, IL-22, IL-28A, IL-18B, and IL-29), which is essential for maintaining the integrity and homeostasis of tissue epithelial layers. By promoting innate immune response, members of this family can limit the damage caused by viral and bacterial infections. In infection or inflammation, some of these cytokines can promote tissue healing. IL10, as a cytokine inhibitory factor (CSIF), reduces inflammation by inhibiting pro-inflammatory cytokines. Thus, IL10 levels in biological samples are closely associated with viral, bacterial infections, or injuries. The present kit is designed to detect bIL10 in bovine serum, plasma, and cell culture supernatants.

AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

Specifications

Application
Protein Analysis & Detection
Assay Points
100
Assay Target
IL10
Assay Target Class
Cytokine
Automation Compatible
Yes
Brand
AlphaLISA
Detection Method
Alpha
Format
Microplates
One Unit Contains
100.0 assay points
Shipping Conditions
Shipped in Blue Ice
Therapeutic Area
Inflammation
Unit Size
100 Assay Points

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Application Note
Antigen-stimulated PBMC cytokine release measured by AlphaLISA bovine cytokine kits

Leptospirosis is an infectious disease caused by Leptospira bacteria, which can spread across different species of mammals...

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