AlphaLISA Bovine IL-17A Detection Kit, 100 Assay Points

Formats:

• Our 100 assay point kit allows you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
• Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
• Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features:

• No-wash steps, no separation steps
• ELISA alternative technology
• Sensitive detection
• Broad sample compatibility
• Small sample volume
• Results in less than 3 hours
• Half the time of an ELISA assay

Interleukin 17A (IL17A) is a 155 aa protein with a unique structure only shared with interleukin 17F. IL17A acts as an early response component in inflammation. Upon detection of an abnormal element, macrophages will secrete interleukin 17A. This cytokine plays two major roles. The first is as a potent chemoattractant for neutrophils and NK killer cells. Its second role is to stimulate the secretion of inflammatory cytokines such as TGFβ, TNFα, IL2 and IL6 amongst others. These roles make IL17A a clear marker of inflammation in animals and also serve to measure early response to infection and/or vaccination. This kit has been designed for the detection of bovine IL17A in serum and cell culture supernatants.

AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.