Skip to main content

AlphaLISA Anti-Bovine IgA Acceptor Beads, 250 ug

AlphaLISA Acceptor beads conjugated to anti-bovine IgA antibody. These beads can be used to capture bovine IgA antibodies.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Product Variants
Part number: AL169C
Unit Size: 250 µg
List price: USD 1,254.00
Your price:
USD 1,254.00
USD 1,254.00 /each
Part number: AL169M
Unit Size: 5 mg
List price: USD 9,546.00
Your price:
USD 0.00
USD 9,546.00 /each
Part number: AL169R
Unit Size: 25 mg
List price: USD 42,490.00
Your price:
USD 0.00
USD 42,490.00 /each

Overview

AlphaLISA Acceptor beads conjugated to anti-bovine IgA antibody. These beads can be used to capture bovine IgA antibodies. These beads can be used in conjunction with Alpha Donor beads to create AlphaLISA no-wash immunoassays for:

  • Antibody-antigen binding studies
  • Analyte detection assays
  • Biomarker detection assays
  • Antibody detection assays
  • Other immunoassays

In a typical AlphaLISA assay, 1 mg of AlphaLISA Acceptor beads is sufficient to run 1,000-2,000 wells using a 50 µL final reaction volume. Bead concentration can be adjusted for optimal performance.

AlphaScreen® and AlphaLISA® are bead-based assay technologies used to study biomolecular interactions in a microplate format. The acronym "Alpha" stands for amplified luminescent proximity homogeneous assay. As the name implies, some of the key features of these technologies are that they are non-radioactive, homogeneous proximity assays. Binding of molecules captured on the beads leads to an energy transfer from one bead to the other, ultimately producing a luminescent/fluorescent signal. To understand how a signal is produced, one must begin with an understanding of the beads. AlphaScreen and AlphaLISA assays require two bead types: Donor beads and Acceptor beads. Each bead type contains a different proprietary mixture of chemicals, which are key elements of the AlphaScreen technology. Donor beads contain a photosensitizer, phthalocyanine, which converts ambient oxygen to an excited and reactive form of O2, singlet oxygen, upon illumination at 680 nm. Please note that singlet oxygen is not a radical; it is molecular oxygen with a single excited electron. Like other excited molecules, singlet oxygen has a limited lifetime prior to falling back to ground state. Within its 4 µsec half-life, singlet oxygen can diffuse approximately 200 nm in solution. If an Acceptor bead is within that proximity, energy is transferred from the singlet oxygen to thioxene derivatives within the Acceptor bead, subsequently culminating in light production at 520-620 nm (AlphaScreen) or at 615 nm (AlphaLISA). In the absence of an Acceptor bead, singlet oxygen falls to ground state and no signal is produced. This proximity-dependent chemical energy transfer is the basis for AlphaScreen's homogeneous nature.

Specifications

Antibody Conjugates
Anti-bovine IgA
Application
Antibody binding
Isotyping
Protein Analysis & Detection
Automation Compatible
Yes
Bead Type or Material
AlphaLISA Acceptor
Brand
AlphaLISA
Detection Method
Alpha
Experimental Type
In vitro
Format
Microplates
Quantity in a Package Amount
1.0 Units
Species
Rat
Shipping Conditions
Shipped in Blue Ice
Unit Size
250 µg

Video gallery

Resources

1-6 of 6 Resources
Application Note Icon Image
Application Note
A Fast and Simple Chemiluminescent Assay for Monitoring of DNA-Protein Interactions

This note describes development of an AlphaLISA immunoassay to monitor the presence of specific DNA-binding proteins in nuclear...

Guide
Alpha Protein-Protein Interaction Quick Start Guide

Alpha has been used to study a wide variety of interactions, including protein:protein, protein:peptide, protein:DNA, protein:RNA...

Guide
Alpha Protein-Protein Interaction Quick Start Guide

Alpha has been used to study a wide variety of interactions, including protein:protein, protein:peptide, protein:DNA, protein:RNA...

Application Note Icon Image
Application Note
Biochemical binding ADCC assays utilizing AlphaLISA toolbox reagents for the characterization of hIgGs and FcγR1A.

Fc Gamma Receptors (FcγRs) are members of the immunoglobulin superfamily and play a critical role in the function of therapeutic...

Guide
ELISA to AlphaLISA Immunoassay Conversion Guide

This guide presents the simple conversion of an ELISA or other immunoassay to an AlphaLISA® immunoassay.

Guide
ELISA to AlphaLISA Immunoassay Conversion Guide

This guide presents the simple conversion of an ELISA or other immunoassay to an AlphaLISA® immunoassay.

SDS, COAs, Manuals and more Illuminator

SDS, COAs, Manuals and more

Are you looking for technical documents for this product. We have housed them in a dedicated section., click on the links below to explore.

Scroll Icon