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AlphaLISA Anti-acetyl-p53 Lysine 382 (p53K382ac) Acceptor Beads, 250 ug

AlphaLISA® Acceptor beads conjugated to an antibody against human p53 that is acetylated at lysine 382 (p53K382ac). These beads can be used for no-wash AlphaLISA epigenetic writer and eraser assays.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Part Number: AL124C
Unit Size: 250 µg
Part Number: AL124M
Unit Size: 5 mg
Part Number: AL124R
Unit Size: 25 mg
  • AlphaLISA® Acceptor beads designed to detect human p53 that is acetylated at lysine 382 (p53K382ac) in a homogeneous AlphaLISA assay. Broad species cross-reactivity is expected based on sequence similarity. Source of antibody: monoclonal.

    The anti-acetyl-p53 Lysine 382 (p53K382ac) AlphaLISA Acceptor beads were used for the development and optimization of a SIRT1 deacetylase assay using a biotinylated p53 (368-393) peptide acetylated at lysine 382 as substrate. A technical note describing the assay is available in our product literature.

    Features:

    • No-wash epigenetic assay
    • Fully-validated mark specificity
    • Substrate flexibility (peptide, protein, histone, nucleosome substrates)
    • Easy-to-automate
    • Fast assay optimization

    AlphaScreen® and AlphaLISA® are bead-based assay technologies used to study biomolecular interactions in a microplate format. The acronym "Alpha" stands for amplified luminescent proximity homogeneous assay. As the name implies, some of the key features of these technologies are that they are non-radioactive, homogeneous proximity assays. Binding of molecules captured on the beads leads to an energy transfer from one bead to the other, ultimately producing a luminescent/fluorescent signal. To understand how a signal is produced, one must begin with an understanding of the beads. AlphaScreen and AlphaLISA assays require two bead types: Donor beads and Acceptor beads. Each bead type contains a different proprietary mixture of chemicals, which are key elements of the AlphaScreen technology. Donor beads contain a photosensitizer, phthalocyanine, which converts ambient oxygen to an excited and reactive form of O2, singlet oxygen, upon illumination at 680 nm. Please note that singlet oxygen is not a radical; it is molecular oxygen with a single excited electron. Like other excited molecules, singlet oxygen has a limited lifetime prior to falling back to ground state. Within its 4 µsec half-life, singlet oxygen can diffuse approximately 200 nm in solution. If an Acceptor bead is within that proximity, energy is transferred from the singlet oxygen to thioxene derivatives within the Acceptor bead, subsequently culminating in light production at 520-620 nm (AlphaScreen) or at 615 nm (AlphaLISA). In the absence of an Acceptor bead, singlet oxygen falls to ground state and no signal is produced. This proximity-dependent chemical energy transfer is the basis for AlphaScreen's homogeneous nature.

  • Antibody Conjugates
    Anti-p53K382ac
    Application
    Epigenetics
    Automation Compatible
    Yes
    Bead Type or Core Bead Type
    AlphaLISA Acceptor
    Brand
    AlphaLISA
    Detection Method
    Alpha
    Experimental Type
    In vitro
    Format
    Microplates
    Molecular Modification
    Acetylation
    Species
    Human
    Shipping Conditions
    Shipped in Blue Ice
    Unit Size
    250 µg

Resources

1-2 of 2 Resources
Guide
Alpha Protein-Protein Interaction Quick Start Guide

Alpha has been used to study a wide variety of interactions, including protein:protein, protein:peptide, protein:DNA, protein:RNA...

Guide
Alpha Protein-Protein Interaction Quick Start Guide

Alpha has been used to study a wide variety of interactions, including protein:protein, protein:peptide, protein:DNA, protein:RNA...