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AlphaLISA Anti-HRP Acceptor Beads, 250 µg

AlphaLISA® Acceptor beads conjugated to anti-HRP antibody. These beads can be used to capture HRP-fusion proteins for AlphaLISA no-wash assays.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).

Product Variants
Part number: AL171C
Unit Size: 250 µg
List price: USD 897.94
Your price:
USD 897.94
USD 897.94 /each
Part number: AL171M
Unit Size: 5 mg
List price: USD 9,127.00
Your price:
USD 0.00
USD 9,127.00 /each
Part number: AL171R
Unit Size: 25 mg
List price: USD 36,820.00
Your price:
USD 0.00
USD 36,820.00 /each

Overview

These beads can be used in conjunction with Alpha Donor beads to create AlphaLISA no-wash immunoassays for:

  • Protein-protein interaction assays
  • Analyte detection assays
  • Biomarker detection assays
  • Other immunoassays

In a typical AlphaLISA assay, 1 mg of Acceptor beads is sufficient to run 1,000-2,000 wells using a 50 µL reaction volume.

Features:

  • No-wash steps, no separation steps
  • Ease-of-use: few addition steps, fast assay development
  • Broad range of affinities: detect strong or weak interactions, from pM to mM affinity
  • Distance: measure very large protein or antibody complexes – spanning up to 200 nm or more
  • High avidity: multiple binding sites on each bead enables use of nanomolar concentrations of antibodies or proteins, as well as use of low affinity binders

AlphaScreen® and AlphaLISA® are bead-based assay technologies used to study biomolecular interactions in a microplate format. The acronym "Alpha" stands for amplified luminescent proximity homogeneous assay. As the name implies, some of the key features of these technologies are that they are non-radioactive, homogeneous proximity assays. Binding of molecules captured on the beads leads to an energy transfer from one bead to the other, ultimately producing a luminescent/fluorescent signal. To understand how a signal is produced, one must begin with an understanding of the beads. AlphaScreen and AlphaLISA assays require two bead types: Donor beads and Acceptor beads. Each bead type contains a different proprietary mixture of chemicals, which are key elements of the AlphaScreen technology. Donor beads contain a photosensitizer, phthalocyanine, which converts ambient oxygen to an excited and reactive form of O2, singlet oxygen, upon illumination at 680 nm. Please note that singlet oxygen is not a radical; it is molecular oxygen with a single excited electron. Like other excited molecules, singlet oxygen has a limited lifetime prior to falling back to ground state. Within its 4 µsec half-life, singlet oxygen can diffuse approximately 200 nm in solution. If an Acceptor bead is within that proximity, energy is transferred from the singlet oxygen to thioxene derivatives within the Acceptor bead, subsequently culminating in light production at 520-620 nm (AlphaScreen) or at 615 nm (AlphaLISA). In the absence of an Acceptor bead, singlet oxygen falls to ground state and no signal is produced. This proximity-dependent chemical energy transfer is the basis for AlphaScreen's homogeneous nature.

Specifications

Antibody Conjugates
Anti-HRP
Application
Protein-protein interactions
Protein Analysis & Detection
Automation Compatible
Yes
Bead Type or Material
AlphaLISA Acceptor
Brand
AlphaLISA
Detection Method
Alpha
Experimental Type
In vitro
Format
Microplates
Unit Size
250 µg

Video gallery

Resources

1-3 of 3 Resources
Application Note Icon
Application Note
A Fast and Simple Chemiluminescent Assay for Monitoring of DNA-Protein Interactions

This note describes development of an AlphaLISA immunoassay to monitor the presence of specific DNA-binding proteins in nuclear...

Guide Icon
Guide
Alpha Protein-Protein Interaction Quick Start Guide

Alpha has been used to study a wide variety of interactions, including protein:protein, protein:peptide, protein:DNA, protein:RNA...

Application Note Icon
Application Note
Biochemical binding ADCC assays utilizing AlphaLISA toolbox reagents for the characterization of hIgGs and FcγR1A.

Fc Gamma Receptors (FcγRs) are members of the immunoglobulin superfamily and play a critical role in the function of therapeutic...

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