Skip to main content
Menu
US

AlphaLISA Anti-Human IgE Acceptor Beads, 250 µg

AlphaLISA® Acceptor beads conjugated to anti-human IgE antibody. These beads can be used in conjunction with Alpha Donor beads to capture human IgE antibodies in no-wash AlphaLISA immunoassays.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).

Product Variants
Part number: AL176C
Unit Size: 250 µg
List price: USD 1,026.00
Your price:
USD 1,026.00
USD 1,026.00 /each
Part number: AL176M
Unit Size: 5 mg
List price: USD 9,441.00
Your price:
USD 0.00
USD 9,441.00 /each
Part number: AL176R
Unit Size: 25 mg
List price: USD 37,870.00
Your price:
USD 0.00
USD 37,870.00 /each

Overview

AlphaLISA Acceptor beads conjugated to anti-human IgE antibody. These beads can be used to capture human IgE antibodies in developing your own AlphaLISA assay for:

  • Antibody-antigen binding studies
  • Analyte detection assays
  • Biomarker detection assays
  • Antibody detection assays
  • Other immunoassays

In a typical AlphaLISA assay, 1 mg of AlphaLISA Acceptor beads is sufficient to run 1,000-2,000 wells using a 50 µL final reaction volume. Bead concentration can be adjusted for optimal performance.

AlphaScreen® and AlphaLISA are bead-based assay technologies used to study biomolecular interactions in a microplate format. The acronym "Alpha" stands for amplified luminescent proximity homogeneous assay. As the name implies, some of the key features of these technologies are that they are non-radioactive, homogeneous proximity assays. Binding of molecules captured on the beads leads to an energy transfer from one bead to the other, ultimately producing a luminescent/fluorescent signal. To understand how a signal is produced, one must begin with an understanding of the beads. AlphaScreen and AlphaLISA assays require two bead types: Donor beads and Acceptor beads. Each bead type contains a different proprietary mixture of chemicals, which are key elements of the AlphaScreen technology. Donor beads contain a photosensitizer, phthalocyanine, which converts ambient oxygen to an excited and reactive form of O2, singlet oxygen, upon illumination at 680 nm. Please note that singlet oxygen is not a radical; it is molecular oxygen with a single excited electron. Like other excited molecules, singlet oxygen has a limited lifetime prior to falling back to ground state. Within its 4 µsec half-life, singlet oxygen can diffuse approximately 200 nm in solution. If an Acceptor bead is within that proximity, energy is transferred from the singlet oxygen to thioxene derivatives within the Acceptor bead, subsequently culminating in light production at 520-620 nm (AlphaScreen) or at 615 nm (AlphaLISA). In the absence of an Acceptor bead, singlet oxygen falls to ground state and no signal is produced. This proximity-dependent chemical energy transfer is the basis for AlphaScreen's homogeneous nature.

Specifications

Antibody Conjugates
Anti-human IgE
Application
Antibody binding
Protein Analysis & Detection
Automation Compatible
Yes
Bead Type or Material
AlphaLISA Acceptor
Brand
AlphaLISA
Detection Method
Alpha
Shipping Conditions
Shipped in Blue Ice
Unit Size
250 µg

Video gallery

SDS, COAs, Manuals and more Illuminator

SDS, COAs, Manuals and more

Are you looking for technical documents for this product. We have housed them in a dedicated section., click on the links below to explore.

Scroll Icon