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AlphaLISA Human ANGPTL4 Detection Kit, 100 Assay Points

The AlphaLISA™ immunoassay kit for human ANGPTL4 enables the quantitative determination of ANGPTL4 in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).

Product Variants
Part number: AL3017HV
Unit Size: 100 Assay Points
List price: USD 680.80
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USD 680.80
USD 680.80 /each
Part number: AL3017C
Unit Size: 500 Assay Points
List price: USD 2,256.00
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USD 0.00
USD 2,256.00 /each
Part number: AL3017F
Unit Size: 5,000 Assay Points
List price: USD 14,900.00
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USD 0.00
USD 14,900.00 /each

Overview

Formats:

  • Our 100 assay point kit allows you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features:

  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Small sample volume
  • Results in less than 3 hours
  • Half the time of an ELISA assay

Angiopoietin-like 4 (ANGPTL4), also known as HFARP, PGAR, or FIAF, is a 55 kDa glycoprotein that is structurally related to the angiopoietin family. It is secreted by the liver and fat tissue, and contains an N-terminal coiled-coil domain, and taC-terminal fibrinogen-like domain. Angiopoietin-like 4 plays a role in lipoprotein metabolism and angiogenesis. It is also involved in numerous cancers and is implicated in the metastatic process by modulating vascular permeability, cancer cell motility and invasiveness. This kit has been designed for the detection of Human Angiopoietin-like 4 in serum and cell culture supernatants.

AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

Specifications

Application
Protein Analysis & Detection
Assay Points
100
Assay Target
ANGPTL4
Assay Target Class
Biomarker
Protein
Automation Compatible
Yes
Brand
AlphaLISA
Detection Method
Alpha
Format
Microplates
Shipping Conditions
Shipped in Blue Ice
Therapeutic Area
Oncology
Unit Size
100 Assay Points

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