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AlphaLISA AAV9 Capsid Detection Kit, 5,000 Assay Points

The AlphaLISA® immunoassay kit for AAV9 enables the quantitative determination of AAV9 capsids in buffer, cell culture media, cell lysates and cell supernatants using a homogeneous AlphaLISA assay (no wash steps).

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Part Number: AL3182HV
Unit Size: 100 Assay Points
Part Number: AL3182C
Unit Size: 500 Assay Points
Part Number: AL3182F
Unit Size: 5,000 Assay Points
  • Adeno-associated virus (AA) vectors are the leading platform for gene delivery for the treatment of a variety of human diseases. AAV Serotype 9 used as a vector in gene therapy has been particularly developed for the treatment of neurological diseases mainly because of its ability to cross the blood-brain barrier to target the central nervous system (astrocytes and neurons). It also exhibits a tropism for other tissues such as liver, muscle, or lung. The AAV9 kit is designed to detect and quantify AAV9 particles.

    Formats
    • Our 100 assay point kit allows you to run 100 wells in 96-well format, using a 50 µL reaction volume (10 µL of sample).
    • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (10 µL of sample).
    • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (10 µL of sample).
    Features
    • No-wash steps, no separation steps
    • ELISA alternative technology
    • Sensitive detection
    • Broad sample compatibility
    • Small sample volume
    • Results in less than 3 hours
    • Half the time of an ELISA assay

    AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is captured by AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

  • Application
    Gene Therapy
    Assay Points
    5000
    Assay Technology
    Alpha
    Automation Compatible
    Yes
    Brand
    AlphaLISA
    Detection Method
    Alpha
    Experimental Type
    In vitro
    Shipping Conditions
    Shipped in Blue Ice
    Unit Size
    5,000 Assay Points

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Whitepaper
Complete gene therapy review in a single industry report

With the increased understanding of molecular and cellular medicine, more specific and efficient gene transfer vectors are now...