The AlphaLISA™ SureFire® Ultra™ Human Total XBP1s assay is a sandwich immunoassay for quantitative detection of total XBP1s in cellular lysates using Alpha Technology.
Feature | Specification |
---|---|
Application | Cell Signaling |
Sample Volume | 10 µL |
The AlphaLISA™ SureFire® Ultra™ Human Total XBP1s assay is a sandwich immunoassay for quantitative detection of total XBP1s in cellular lysates using Alpha Technology.
The spliced form of X-box binding protein 1 (XBP1s) is an active transcription factor that plays a vital role in the unfolded protein response (UPR). In response to endoplasmic reticulum stress and UPR, BiP relaxes its inhibitory binding to trigger IRE1. After dimerizing and auto-phosphorylating, the IRE1 receptor initiates its mRNase activity. In order to facilitate XBP-1u mRNA's translation and subsequent translocation to the nucleus, IRE1 splices it into XBP1s. Afterward, XBP-1 functions as a transcription factor. XBP1s is an important therapeutic target in studying various diseases, including cancer, diabetes, and autoimmune and inflammatory diseases.
The AlphaLISA SureFire Ultra Human Total XBP1s Detection Kit is a sandwich immunoassay for the quantitative detection of total XBP1s in cellular lysates, using Alpha Technology.
Formats:
AlphaLISA SureFire Ultra kits are compatible with:
Alpha SureFire kits can be used for:
Application |
Cell Signaling
|
---|---|
Automation Compatible |
Yes
|
Brand |
AlphaLISA SureFire Ultra
|
Detection Modality |
Alpha
|
Lysis Buffer Compatibility |
Lysis Buffer B
|
Molecular Modification |
Total
|
Predicted Species Reactivity |
Human
|
Product Group |
Kit
|
Sample Volume |
10 µL
|
Shipping Conditions |
Shipped in Blue Ice
|
Target |
XBP1s
|
Target Class |
Phosphoproteins
|
Target Species |
Human
|
Technology |
Alpha
|
Unit Size |
500 assay points
|
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