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AlphaLISA Surefire Ultra Human Phospho-VEGFR2 (Tyr1175) Detection Kit, 500 Assay Points
The AlphaLISA® SureFire® Ultra™p-VEGF Receptor 2 (Tyr1175) assay is a sandwich immunoassay for quantitative detection of phospho-VEGFR Receptor 2 (phosphorylated on Tyr1175) in cellular lysates using Alpha Technology.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Product information
Overview
The AlphaLISA®SureFire®Ultra™ p-VEGF Receptor 2 (Tyr1175) assay is a sandwich immunoassay for quantitative detection of phospho-VEGF Receptor 2 (phosphorylated on Tyr1175) in cellular lysates using Alpha Technology.
In the AlphaLISA®SureFire®Ultra™ assay, Donor beads are coated with streptavidin to capture one of the antibodies, which is biotinylated. Acceptor beads are coated with a proprietary CaptSure™ agent that immobilizes the other antibody, labeled with a CaptSure™ tag. In the presence of phosphorylated protein, the two antibodies bring the Donor and Acceptor beads close together, generating signal. The amount of light emission is directly proportional to the amount of phosphoprotein present in the sample.
AlphaLISA®SureFire®Ultra™ kits are compatible with:
- Cell and tissue lysates
- Antibody modulators
- Biotherapeutic antibodies
Alpha SureFire® kits can be used for:
- Cellular kinase assays
- Receptor activation studies
- Screening
Specifications
| Assay Pathway |
Receptor signaling
|
|---|---|
| Assay Points |
500
|
| Automation Compatible |
Yes
|
| Brand |
AlphaLISA SureFire Ultra
|
| Detection Method |
Alpha
|
| Experimental Type |
In vitro
|
| Format |
Microplates
|
| One Unit Contains |
500.0 assay points
|
| Quantity in a Package Amount |
1.0 per pack
|
| Shipping Conditions |
Shipped in Blue Ice
|
| Unit Size |
500 assay points
|
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