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AlphaLISA SureFire Ultra Human Phospho-ULK1 (Ser758) Detection Kit, 50,000 Assay Points

The AlphaLISA® SureFire® Ultra Human Phospho-ULK1 (Ser758) assay is a sandwich immunoassay for quantitative detection of phospho-ULK1 (Ser758) in cellular lysates using Alpha Technology.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Part Number: ALSU-PULK1-B500
Unit Size: 500 Assay Points
Part Number: ALSU-PULK1-B10K
Unit Size: 10,000 Assay Points
Part Number: ALSU-PULK1-B50K
Unit Size: 50,000 Assay Points
Part Number: ALSU-PULK1-B-HV
Unit Size: 100 Assay Points
  • UNC-51 like autophagy activating kinase exists in two similar isoforms, ULK1 and ULK2. ULK1 is regulated by the key nutrient/energy-sensitive kinases mTOR and AMPK, which are both able to phosphorylate ULK1 and directly regulate its kinase activity. ULK1 forms a complex with the Atg13 and FIP200 proteins, this complex is the most upstream component of the core autophagy machinery and is, therefore, the key initiator of autophagy in mammalian cells. ULK1 is implicated in a variety of therapeutic areas including oncology, autoimmunity, neurology, and diabetes.

    The AlphaLISA SureFire Human Phospho-ULK1 (Ser758) Detection Kit is a sandwich immunoassay for the quantitative detection of phospho-ULK1 (Ser758) in cellular lysates, using Alpha Technology.

    Formats
    • The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
    • The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
    • The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
    • The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.
    AlphaLISA SureFire Ultra kits are compatible with
    • Cell and tissue lysates
    • Antibody modulators
    • Biotherapeutic antibodies
    Alpha SureFire kits can be used for
    • Cellular kinase assays
    • Receptor activation studies
    • Screening
  • Application
    Protein Analysis & Detection
    Protein Detection
    Assay Points
    50000
    Assay Target Class
    Phospho-protein
    Assay Technology
    Alpha
    Automation Compatible
    Yes
    Brand
    AlphaLISA SureFire Ultra
    Detection Method
    Alpha
    Experimental Type
    In vitro
    Shipping Conditions
    Shipped in Blue Ice
    Target Species
    Human
    Therapeutic Area
    Autoimmunity
    Immuno-oncology
    Metabolic
    Neuroscience
    Oncology
    Unit Size
    50,000 Assay Points

Resources

1-2 of 2 Resources
Guide
AlphaLISA SureFire Ultra assay optimization

This guide outlines further possible optimization of cellular and immunoassay parameters to ensure the best possible results are...

Application Note Icon Image
Application Note
Characterizing Chemokine Receptor Inhibitors with AlphaLISA SureFire Ultra

Therapeutic antibodies directed to cell surface receptors are increasingly being developed as treatments for a variety of diseases...