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AlphaLISA SureFire Ultra Human Phospho-Ubiquitin (Ser65) Detection Kit, 50,000 Assay Points

The AlphaLISA® SureFire® Ultra Human Phospho-Ubiquitin (Ser65) assay is a sandwich immunoassay for quantitative detection of phospho-ubiquitin (Ser65) in cellular lysates using Alpha Technology.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Part Number: ALSU-PUBI-A500
Unit Size:
Part Number: ALSU-PUBI-A10K
Unit Size:
Part Number: ALSU-PUBI-A50K
Unit Size:
Part Number: ALSU-PUBI-A-HV
Unit Size:
  • Ubiquitin is a highly conserved regulatory protein found in most tissues of eukaryotic organism. Phosphorylation of ubiquitin is a key step in the process of mitochondria quality control through the Parkin/PINK-1 interaction. Parkin is a ubiquitin ligase E3 that conjugates ubiquitin to impaired-mitochondrial proteins, leading to organelle degradation. PINK1 is a Ser/Thr kinase that accumulates only on impaired mitochondria and phosphorylates two substrates, the ubiquitin-like domain of Parkin and ubiquitin chains. The amplified Parkin/PINK-1 dependent ubiquitin functions act as a signal for mitophagy. Dysregulation of this signaling is linked to neurodegenerative diseases such as Parkinson's Disease.

    The AlphaLISA SureFire Ultra Human Phospho-Ubiquitin (Ser65) assay is a sandwich immunoassay for the quantitative detection of phospho-Ubiquitin (Ser65) in cellular lysates, using Alpha Technology.

    Formats:
    • The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
    • The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
    • The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
    • The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.
    AlphaLISA SureFire Ultra kits are compatible with:
    • Cell and tissue lysates
    • Antibody modulators
    • Biotherapeutic antibodies
    Alpha SureFire kits can be used for:
    • Cellular kinase assays
    • Receptor activation studies
    • Screening
  • Application
    Protein Detection
    Assay Technology
    Alpha
    Automation Compatible
    Yes
    Brand
    AlphaLISA SureFire Ultra
    Detection Method
    Alpha
    Experimental Type
    In vitro
    Target Species
    Human
    Therapeutic Area
    Central Nervous System

Resources

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Application Note
Characterizing Chemokine Receptor Inhibitors with AlphaLISA SureFire Ultra
surefire, neutralizing antibody, neutralizing antibodies, biotherapeutics, biotx