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AlphaLISA Surefire Ultra Total STAT5 Detection Kit, 10,000 Assay Points

The AlphaLISA® SureFire® Ultra™ Total STAT5 assay is a sandwich immunoassay for quantitative detection of STAT5 (both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for phosphorylation studies.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Part Number: ALSU-TST5-A500
Unit Size: 500 Assay Points
Part Number: ALSU-TST5-A10K
Unit Size: 10,000 Assay Points
Part Number: ALSU-TST5-A50K
Unit Size: 50,000 Assay Points
Part Number: ALSU-TST5-A-HV
Unit Size: 100 Assay Points
  • The AlphaLISA® SureFire® Ultra™ Total STAT5 assay is a sandwich immunoassay for quantitative detection of STAT5 (both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for phosphorylation studies.

    The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 µL reaction volume. The 500 point kit contains reagents to run 500 wells in 384-well format, using a 20 µL reaction volume. The 10,000 point kit contains reagents to run 10,000 wells in 384-well format, using a 20 µL reaction volume. The 50,000 point kit contains reagents to run 500 wells in 384-well format, using a 20 µL reaction volume.

    In the AlphaLISA® SureFire® Ultra™ assay, Donor beads are coated with streptavidin to capture one of the antibodies, which is biotinylated. Acceptor beads are coated with a proprietary CaptSure™ agent that immobilizes the other antibody, labeled with a CaptSure™ tag. In the presence of target protein, the two antibodies bring the Donor and Acceptor beads close together, generating signal. The amount of light emission is directly proportional to the amount of protein present in the sample.

    AlphaLISA® SureFire® Ultra™ kits are compatible with:

    • Cell and tissue lysates
    • Antibody modulators
    • Biotherapeutic antibodies

    Alpha SureFire® kits can be used for:

    • Cellular kinase assays
    • Receptor activation studies
    • Screening
  • Application
    Protein Analysis & Detection
    Target Engagement
    Assay Pathway
    JAK/STAT
    Assay Target Class
    Protein
    Brand
    AlphaLISA SureFire Ultra
    Detection Method
    Alpha
    Experimental Type
    In vitro
    Format
    Microplates
    Quantity in a Package Amount
    1.0 Units
    Species
    Human
    Unit Size
    10,000 Assay Points

Resources

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Brochure Icon Image
Brochure
Alpha SureFire Ultra no-wash immunoassay catalog

Discover Alpha SureFire® Ultra™ assays, the no-wash cellular kinase assays leveraging Revvity's exclusive bead-based technology...

Guide
AlphaLISA SureFire Ultra assay optimization

This guide outlines further possible optimization of cellular and immunoassay parameters to ensure the best possible results are...

Application Note Icon Image
Application Note
Characterizing Chemokine Receptor Inhibitors with AlphaLISA SureFire Ultra

Therapeutic antibodies directed to cell surface receptors are increasingly being developed as treatments for a variety of diseases...