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AlphaLISA SureFire Ultra Human Phospho-TIE2 (Tyr992) Detection Kit, 100 Assay Points

The AlphaLISA™ SureFire® Ultra™ Human Phospho-TIE2 (Tyr992) assay is a sandwich immunoassay for quantitative detection of phospho-TIE2 in cellular lysates using Alpha technology.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

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Feature Specification
Application Cell Signaling
Sample Volume 30 µL

The AlphaLISA™ SureFire® Ultra™ Human Phospho-TIE2 (Tyr992) assay is a sandwich immunoassay for quantitative detection of phospho-TIE2 in cellular lysates using Alpha technology.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

Click to copy promo code to clipboard.
SAVE 15% NOW on online orders. Use promo code below.
YEAREND15
Offer valid until 12/15. Terms and conditions apply.
Product Variants
Unit Size: 100 Assay Points
Part #:
ALSU-PTIE2-A-HV
List Price
USD 694.00
Unit Size: 500 Assay Points
Part #:
ALSU-PTIE2-A500
List Price
USD 2,346.00
Unit Size: 10,000 Assay Points
Part #:
ALSU-PTIE2-A10K
List Price
USD 14,270.00
Unit Size: 50,000 Assay Points
Part #:
ALSU-PTIE2-A50K
List Price
USD 46,060.00

Overview

The TEK receptor tyrosine kinase (TIE2), encoded by the TEK gene in humans, is primarily expressed on endothelial cells. Upon binding with angiopoietin-1 (Angpt1), TIE2 induces angiogenesis and supports endothelial cell survival. This activation leads to downstream signaling pathways, including MEK/ERK, IKKb/NFkB, and AKT. Consequently, TIE2 contributes to vascular stability, cellular proliferation, inflammation, and cell survival. Elevated levels of Ang1 and/or Ang2 have been observed in patients with various cancers.

The AlphaLISA SureFire Ultra Human Phospho-TIE2 (Tyr992) Detection Kit is a sandwich immunoassay for the quantitative detection of phospho-TIE2 in cellular lysates, using Alpha technology.

Formats:

  • The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
  • The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
  • The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
  • The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.

AlphaLISA SureFire Ultra kits are compatible with:

  • Cell and tissue lysates
  • Antibody modulators
  • Biotherapeutic antibodies

Alpha SureFire kits can be used for:

  • Cellular kinase assays
  • Receptor activation studies
  • High-throughput drug screening

Specifications

Application
Cell Signaling
Automation Compatible
Yes
Brand
AlphaLISA SureFire Ultra
Detection Modality
Alpha
Lysis Buffer Compatibility
Lysis Buffer
Molecular Modification
Phosphorylation
Product Group
Kit
Sample Volume
30 µL
Shipping Conditions
Shipped in Blue Ice
Target
TIE2
Target Class
Phosphoproteins
Target Species
Human
Technology
Alpha
Therapeutic Area
Oncology
Unit Size
100 Assay Points

Assay validation

Induction of TIE2 Phospho (Tyr992) in HUVEC cells

HUVEC cells were seeded in a 96-well culture plate (20,000 cells/well) in complete medium and incubated for 48 hours at 37°C, 5% CO2. The cells were further treated with increasing concentrations of Pervanadate for 30 minutes.

After treatment, the cells were lysed with 50 µL of lysis buffer for 10 minutes at RT with shaking at 350 rpm. TIE2 Phospho (Tyr992) and Total levels were evaluated using respective AlphaLISA SureFire Ultra assays. For the detection step, 10 µL of cell lysate (approximately 8,000 cells) were transferred into a 384-well white OptiPlate, followed by 5 µL of Acceptor Mix and incubated for 1 hour at RT.  Finally, 5 µL of Donor Mix was then added to each well and incubated for 1 hour at RT in the dark. The plate was read on an Envision Nexus™ using standard AlphaLISA settings.

Induction of TIE2 Phospho (Tyr992) in HUVEC cells

Resources

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