TFE3, or transcription factor E3, is a member of the basic helix-loop-helix leucine zipper MiT transcription factory family. MiT/TFE transcription factors play essential roles in the regulation of autophagy and lysosomal biogenesis. Phosphorylation is the primary regulator of MiT/TFE. The phosphorylated protein remains in the cytoplasm and, following dephosphorylation, translocates to the nucleus, where it induces autophagy and lysosomal biogenesis by promoting the expression of hundreds of genes. Several signaling molecules involved in the mTORC1, PKC, and AKT pathways can directly influence the transcription factor-mediated lysosome-to-nucleus signaling. TFE3 is associated with Parkinson’s disease, Alzheimer’s diseases, and various types of cancer.

The AlphaLISA SureFire Ultra Human and Mouse Phospho-TFE3 (Ser321) is a sandwich immunoassay for the quantitative detection of phospho-TFE3 (Ser321) in cellular lysates, using Alpha Technology.

 Formats:

• The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
• The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
• The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
• The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.

AlphaLISA SureFire Ultra kits are compatible with:

• Cell and tissue lysates
• Antibody modulators
• Biotherapeutic antibodies

Alpha SureFire kits can be used for:

• Cellular kinase assays
• Receptor activation studies
• Screening