Skip to main content
Menu
US

AlphaLISA SureFire Ultra Human Phospho-Tau (Thr217) Detection Kit, 100 Assay Points

The AlphaLISA™ SureFire® Ultra™ Human Phospho-Tau (Thr217) assay is a sandwich immunoassay for quantitative detection of phospho-Tau in cellular lysates using Alpha technology.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

Click to copy promo code to clipboard.
SAVE 15% NOW on online orders. Use promo code below.
YEAREND15
Offer valid until 12/15. Terms and conditions apply.
Feature Specification
Application Cell Signaling
Sample Volume 30 µL

The AlphaLISA™ SureFire® Ultra™ Human Phospho-Tau (Thr217) assay is a sandwich immunoassay for quantitative detection of phospho-Tau in cellular lysates using Alpha technology.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

Click to copy promo code to clipboard.
SAVE 15% NOW on online orders. Use promo code below.
YEAREND15
Offer valid until 12/15. Terms and conditions apply.
Product Variants
Unit Size: 100 Assay Points
Part #:
ALSU-PTAU-G-HV
List Price
USD 694.00
Unit Size: 500 Assay Points
Part #:
ALSU-PTAU-G500
List Price
USD 2,346.00
Unit Size: 10,000 Assay Points
Part #:
ALSU-PTAU-G10K
List Price
USD 14,270.00
Unit Size: 50,000 Assay Points
Part #:
ALSU-PTAU-G50K
List Price
USD 46,060.00

Overview

Tau proteins, derived from tubulin-associated units, play a pivotal role in the pathogenesis of Alzheimer’s disease (AD). Hyperphosphorylated Tau aggregates form filaments, which can further condense into neurofibrillary tangles. These Tau aggregates, often referred to as ‘seeds,’ can propagate pathology from cell to cell, similar to prion transmission. Effective therapeutic strategies for AD include medications that modulate Tau hyperphosphorylation and reduce Tau aggregation.

The AlphaLISA SureFire Ultra Human Phospho-Tau (Thr217) Detection Kit is a sandwich immunoassay for the quantitative detection of phospho-Tau in cellular lysates, using Alpha technology.

Formats:

  • The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
  • The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
  • The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
  • The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.

AlphaLISA SureFire Ultra kits are compatible with:

  • Cell and tissue lysates
  • Antibody modulators
  • Biotherapeutic antibodies

Alpha SureFire kits can be used for:

  • Cellular kinase assays
  • Receptor activation studies
  • High-throughput drug screening

Specifications

Application
Cell Signaling
Automation Compatible
Yes
Brand
AlphaLISA SureFire Ultra
Detection Modality
Alpha
Lysis Buffer Compatibility
Lysis Buffer
Molecular Modification
Phosphorylation
Product Group
Kit
Sample Volume
30 µL
Shipping Conditions
Shipped in Blue Ice
Target
Tau
Target Class
Phosphoproteins
Target Species
Human
Technology
Alpha
Therapeutic Area
Neuroscience
Unit Size
100 Assay Points

Assay validation

Inhibition of phospho-Tau (Thr217) in endogenous cellular models

MCF7 cells were seeded in a 96-well plate(40,000 cells/well) in complete medium, and incubated overnight at 37°C, 5% CO2. The cells were treated for 6 hours with increasing concentrations of Staurosporine prepared in media containing 1% FBS. After treatment, the cells were lysed with 100 µL of lysis buffer for 10 minutes at RT with shaking (350 rpm). Tau Phospho(Thr217) and Total levels were evaluated using respective AlphaLISA SureFire Ultra assays. For the detection step, 10 µL of cell lysate (approximately 4,000 cells) was transferred into a 384-well white OptiPlate, followed by 5 µL of Acceptor mix and incubated for 1 hour at RT. Finally, 5 µL of Donor mix was then added to each well and incubated for 1 hour at RT in the dark. The plate was read on an Envision Nexus™ using standard AlphaLISA settings.

As expected, Staurosporine (a broad-spectrum protein kinase inhibitor) triggered a dose-dependent decrease in the levels of Phospho-Tau (Thr217).

Inhibition of phospho-Tau (Thr217) in endogenous cellular models

SH-SY5Y cells were seeded in a 96-well plate (20,000 cells/well) in complete medium, and incubated for 96 hours at 37°C, 5% CO2. The cells were treated for 6 hours with increasing concentrations of Staurosporine prepared in media containing 1% FBS.

After treatment, the cells were lysed with 100 µL of lysis buffer for 10 minutes at RT with shaking (350 rpm). Tau Phospho(Thr217) and Total levels were evaluated using respective AlphaLISA SureFire Ultra assays. For the detection step, 10 µL of cell lysate (approximately 4,000 cells) was transferred into a 384-well white OptiPlate, followed by 5 µL of Acceptor mix and incubated for 1 hour at RT. Finally, 5 µL of Donor mix was then added to each well and incubated for 1 hour at RT in the dark. The plate was read on an Envision Nexus using standard AlphaLISA settings.

Inhibition of phospho-Tau (Thr217) in endogenous cellular models

 

Resources

Are you looking for resources, click on the resource type to explore further.

1-5 of 5 Resources
Technical Note Icon
Technical Note
AlphaLISA Acetyl‑Histone H3 lysine 27 (H3K27ac) cellular detection kit

Quantifying H3K27ac Levels with AlphaLISA Assay

In this technical note, you will discover how AlphaLISA immunodetection assay...

Technical Note Icon
Technical Note
AlphaLISA Di-Methyl-Histone H3 lysine 4 (H3K4me2) cellular assay

Decoding Histone Modifications: A Targeted Assay for H3K4me2

In this study, you will find details about an optimized assay designed...

Technical Note Icon
Technical Note
AlphaLISA tri-methyl-Histone H3 lysine 27 (H3K27me3) cellular detection kit

Homogeneous Assay for Tri-Methylated Histone H3 Lysine 27 in Cellular Extracts

In this technical note discover how AlphaLISA assay...

Technical Note Icon
Technical Note
Quantifying TNFR1 in both soluble and membrane bound form using AlphaLISA technology

Quantitative Assessment of Soluble TNFR1 Using the Human TNFR1 AlphaLISA Kit

This technical note provides additional evidence of...

Technical Note Icon
Technical Note
Quantitative analysis of lysine 36 demethylation with AlphaLISA

Assessing H3K36 Demethylation Using AlphaLISA Assay

This technical note gives you details about how AlphaLISA immunodetection...

Scroll Icon