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AlphaLISA SureFire Ultra Human and Mouse Phospho-TAK1 (Thr184/187) Detection Kit, 100 Assay Points

The AlphaLISA® SureFire® Ultra Human and Mouse Phospho-TAK1 (Thr184/187) assay is a sandwich immunoassay for quantitative detection of phospho-TAK1 (Thr184/187) in cellular lysates using Alpha Technology.

Part Number: ALSU-PTAK-A-HV
Unit Size: 100 Assay Points
Part Number: ALSU-PTAK-A500
Unit Size: 500 Assay Points
Part Number: ALSU-PTAK-A50K
Unit Size: 50,000 Assay Points
Part Number: ALSU-PTAK-A10K
Unit Size: 10,000 Assay Points
  • Transforming growth factor β-activated kinase 1 (TAK1) is a member of the MAPK kinase kinase (MAPKKK) family and a key regulator of the innate immunity and the proinflammatory signaling pathways. TAK1 facilitates the activation of the nuclear factor B (NF-κB), c-Jun N-terminal kinase (JNK), and p38 pathways in response to the inflammatory proteins interleukin-1 (IL-1), tumor necrosis factor (TNF), and toll-like receptor (TLR) agonists. In addition, TAK1 mediates the signaling from T- and B-cell receptors, which is a crucial component of adaptive immunity. TAK1 is associated with various autoimmune diseases (rheumatoid arthritis, inflammatory bowel syndrome, kidney disease, skin inflammation, chronic pain) and cancers (mantle cell lymphoma, breast cancer, colon cancer).

    The AlphaLISA SureFire Human and Mouse Phospho-TAK1 (Thr184/187) Detection Kit is a sandwich immunoassay for the quantitative detection of phospho-TAK1 (Thr184/187) in cellular lysates, using Alpha Technology.

    Formats:
    • The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
    • The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
    • The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
    • The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.
    AlphaLISA SureFire Ultra kits are compatible with:
    • Cell and tissue lysates
    • Antibody modulators
    • Biotherapeutic antibodies
    Alpha SureFire kits can be used for:
    • Cellular kinase assays
    • Receptor activation studies
    • Screening

    For research use only; not for diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).

  • Automation Compatible
    Yes
    Application
    Protein Analysis & Detection
    Protein Detection
    Assay Target
    TAK1
    Assay Target Class
    Phospho-protein
    Assay Technology
    Alpha
    Detection Method
    Alpha
    Experimental Type
    In vitro
    Target Species
    Human
    Mouse
    Therapeutic Area
    Autoimmunity
    Oncology
    Brand
    AlphaLISA SureFire Ultra
    Unit Size
    100 Assay Points