AlphaLISA SureFire Ultra Mouse Total SYK Detection Kit, 500 Assay Points

The cytoplasmic tyrosine/serine kinase known as SYK (Spleen Tyrosine Kinase) is essential for signal transmission, particularly in immune cells such as B cells and microglia (brain-resident macrophages). SYK interacts with the cytoplasmic domains of CD79 subunits or DAP12 when B cell or TREM2/DAP12 receptor complexes are stimulated, respectively, via binding to their distinct phosphorylated receptor motifs, known as "p-ITAMs." SYK is subsequently activated as a result of Tyr525/526 autophosphorylation of its catalytic domain. Due to SYK's subsequent phosphorylation of downstream substrates like BTK, numerous signaling cascades and physiological reactions—including cell activation, proliferation, and survival—are triggered. SYK is implicated in cancer, central nervous system disorders, and autoimmune diseases.

The AlphaLISA SureFire Mouse Total SYK Detection Kit is a sandwich immunoassay for the quantitative detection of total SYK in cellular lysates, using Alpha Technology.

Formats

• The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
• The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
• The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
• The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.

AlphaLISA SureFire Ultra kits are compatible with

• Cell and tissue lysates
• Antibody modulators
• Biotherapeutic antibodies

Alpha SureFire kits can be used for

• Cellular kinase assays
• Receptor activation studies
• Screening