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AlphaLISA Surefire Ultra Human Total STAT4 Detection Kit, 100 Assay Points

The AlphaLISA® SureFire® Ultra™ total STAT4 assay is a sandwich immunoassay for quantitative detection of STAT4 (phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be a normalization phosphorylation studies.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Part Number: ALSU-TST4-A-HV
Unit Size: 100 Assay Points
Part Number: ALSU-TST4-A500
Unit Size: 500 assay points
Part Number: ALSU-TST4-A50K
Unit Size: 50,000 assay points
Part Number: ALSU-TST4-A10K
Unit Size: 10,000 assay points
  • The AlphaLISA SureFire Ultra total STAT4 assay is a sandwich immunoassay for quantitative detection of total STAT4 (phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology.

    Formats:

    • The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
    • The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
    • The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
    • The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.

    In the AlphaLISA SureFire Ultra assay, Donor beads are coated with streptavidin to capture one of the antibodies, which is biotinylated. Acceptor beads are coated with a proprietary CaptSure™ agent that immobilizes the other antibody, labeled with a CaptSure™ tag. In the presence of target protein, the two antibodies bring the Donor and Acceptor beads close together, generating signal. The amount of light emission is directly proportional to the amount of protein present in the sample.

    AlphaLISA SureFire Ultra kits are compatible with:

    • Cell and tissue lysates
    • Antibody modulators
    • Biotherapeutic antibodies

    Alpha SureFire kits can be used for:

    • Cellular kinase assays
    • Receptor activation studies
    • Screening
  • Application
    Protein Analysis & Detection
    Assay Points
    100
    Assay Target
    STAT4
    Assay Target Class
    Protein
    Assay Technology
    Alpha
    Automation Compatible
    Yes
    Brand
    AlphaLISA SureFire Ultra
    Detection Method
    Alpha
    Experimental Type
    In vitro
    Therapeutic Area
    Autoimmunity
    Immuno-oncology
    Inflammation
    Unit Size
    100 Assay Points

Resources

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Application Note
Characterizing Chemokine Receptor Inhibitors with AlphaLISA SureFire Ultra
SureFire, neutralizing antibody, neutralizing antibodies, biotherapeutics, biotx