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AlphaLISA Surefire Ultra Total SQSTM1 p62 Detection Kit, 10,000 Assay Points

The AlphaLISA® SureFire® Ultra Human and Mouse Total SQSTM1 p62 assay is a sandwich immunoassay for quantitative detection of total SQSTM1 p62 in cellular lysates using Alpha Technology.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Part Number: ALSU-TSQSTM-A500
Unit Size:
Part Number: ALSU-TSQSTM-A10K
Unit Size:
Part Number: ALSU-TSQSTM-A50K
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Part Number: ALSU-TSQSTM-A-HV
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  • Sequestome-1 or ubiquitin binding protein p62 (SQSTM1 p62) is an autophagosome cargo protein that targets other proteins that bind to it for selective autophagy. SQSTM1 p62 is an adaptor protein which is initially phosphorylated by ULK1 on Serine 407, then on Serine 403 by casein kinase 2 or TBK1. The phosphorylation on Ser403 increases its affinity for ubiquitin chains, thus enabling SQSTM1 p62 to bind to ubiquitinated cargo proteins. Ubiquitinated proteins or ubiquitin coated mitochondria associated with SQSTM1 p62 proteins are taken away in phagosomes, whose content is cleared out after lysosomal fusion. SQSTM1 p62 is implicated in Alzheimer’s disease, Huntington’s disease, NASH, and numerous types of cancer.

    The AlphaLISA SureFire Ultra Human and Mouse Total SQSTM1 p62 Detection Kit is a sandwich immunoassay for the quantitative detection of total SQSTM1 p62 in cellular lysates, using Alpha Technology.

    Formats:
    • The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
    • The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
    • The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
    • The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.
    AlphaLISA SureFire Ultra kits are compatible with:
    • Cell and tissue lysates
    • Antibody modulators
    • Biotherapeutic antibodies
    Alpha SureFire kits can be used for:
    • Cellular kinase assays
    • Receptor activation studies
    • Screening
  • Application
    Protein Analysis & Detection
    Protein Detection
    Assay Points
    10000
    Assay Target Class
    Phospho-protein
    Assay Technology
    Alpha
    Automation Compatible
    Yes
    Brand
    AlphaLISA SureFire Ultra
    Detection Method
    Alpha
    Experimental Type
    In vitro
    Target Species
    Human
    Mouse
    Therapeutic Area
    Neuroscience
    Oncology

Resources

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Application Note
Characterizing Chemokine Receptor Inhibitors with AlphaLISA SureFire Ultra
surefire, neutralizing antibody, neutralizing antibodies, biotherapeutics, biotx