Sequestome-1, also known as ubiquitin binding protein p62 or SQSTM1 p62, is an autophagosome cargo protein that directs selective autophagy toward proteins that bind to it. SQSTM1 p62 is an adaptor protein that can attach to cargo proteins that have been ubiquitinated because it has a higher affinity for ubiquitin chains post-phosphorylation. After lysosomal fusion, the contents of phagosomes are eliminated, removing ubiquitinated proteins or mitochondria associated with SQSTM1 p62 proteins. Alzheimer's disease, Huntington's disease, NASH, and many cancers are all linked to SQSTM1 p62.

The AlphaLISA SureFire Human and Mouse Phospho-SQSTM1 p62 (Ser403) Detection Kit is a sandwich immunoassay for the quantitative detection of phospho-SQSTM1 p62 (Ser403) in cellular lysates, using Alpha Technology.

Formats

• The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
• The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
• The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
• The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.

AlphaLISA SureFire Ultra kits are compatible with

• Cell and tissue lysates
• Antibody modulators
• Biotherapeutic antibodies

Alpha SureFire kits can be used for

• Cellular kinase assays
• Receptor activation studies
• Screening