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AlphaLISA Surefire Ultra Total SMAD4 Detection Kit, 50,000 Assay Points

The AlphaLISA® SureFire® Ultra Human and Mouse Total SMAD4 assay is a sandwich immunoassay for quantitative detection of total SMAD4 in cellular lysates using Alpha Technology.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Part Number: ALSU-TSM4-A50K
Unit Size: 50,000 Assay Points
Part Number: ALSU-TSM4-A-HV
Unit Size: 100 Assay Points
Part Number: ALSU-TSM4-A10K
Unit Size: 10,000 Assay Points
Part Number: ALSU-TSM4-A500
Unit Size: 500 Assay Points
  • SMAD family member 4 (SMAD4), also known as mothers against decapentaplegic homolog 4, or DPC4 (deleted in pancreatic cancer-4), belongs to the SMAD family of transcription factor proteins, which act as mediators of TGF-β signal transduction. In conjunction with SMAD 2 and SMAD 3, the SMAD2/3/4 complex forms and acts as a transcription factor that promotes the expression of a pro-fibrotic phenotype. Mutated SMAD4 is found in many types of cancers and fibrosis.

    The AlphaLISA SureFire Ultra Human and Mouse Total SMAD4 Detection Kit is a sandwich immunoassay for the quantitative detection of total SMAD4 in cellular lysates, using Alpha Technology.

    Formats
    • The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
    • The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
    • The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
    • The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.
    AlphaLISA SureFire Ultra kits are compatible with
    • Cell and tissue lysates
    • Antibody modulators
    • Biotherapeutic antibodies
    Alpha SureFire kits can be used for
    • Cellular kinase assays
    • Receptor activation studies
    • Screening
  • Application
    Protein Analysis & Detection
    Protein Detection
    Assay Points
    50000
    Assay Target
    SMAD4
    Assay Target Class
    Phospho-protein
    Assay Technology
    Alpha
    Automation Compatible
    Yes
    Brand
    AlphaLISA SureFire Ultra
    Detection Method
    Alpha
    Experimental Type
    In vitro
    Target Species
    Human
    Mouse
    Therapeutic Area
    Metabolic
    Oncology
    Unit Size
    50,000 Assay Points

Resources

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Application Note Icon Image
Application Note
Characterizing Chemokine Receptor Inhibitors with AlphaLISA SureFire Ultra
SureFire, neutralizing antibody, neutralizing antibodies, biotherapeutics, biotx