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Extra control lysate for the AlphaLISA® SureFire® Ultra™ Total SLP76 assay. This control is already provided in the AlphaLISA SureFire Ultra Total SLP76 assay kit, but can also be ordered separately.
This control lysate is intended for use with the AlphaLISA SureFire Ultra Total SLP76 Detection Kit or for use as a control for those running orthogonal or secondary assays along with the SureFire assay. The lysate is prepared from Jurkat cells, cultured in T175 flask to 106 cells/mL in 10% FBS containing medium, then treated with 11 mM H2O2 and 50 nM Calyculin A for 10 minutes and lysed at 3.2X106 cells/mL with 1X SureFire Ultra Ultra Lysis Buffer.
AlphaLISA SureFire Ultra