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AlphaLISA Surefire Ultra Human Phospho-SLP76 (Tyr113) Detection Kit, 100 Assay Points

The AlphaLISA® SureFire® Ultra p-SLP76 (Tyr113) assay is a sandwich immunoassay for quantitative detection of phospho-SLP76 (phosphorylated on Tyr113) in cellular lysates using Alpha Technology.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).

Product Variants
Part number: ALSU-PSLP-B500
Unit Size: 500 Assay Points
List price: USD 2,230.00
Your price:
USD 0.00
USD 2,230.00 /each
Part number: ALSU-PSLP-B10K
Unit Size: 10,000 Assay Points
List price: USD 13,540.00
Your price:
USD 0.00
USD 13,540.00 /each
Part number: ALSU-PSLP-B50K
Unit Size: 50,000 Assay Points
List price: USD 43,750.00
Your price:
USD 0.00
USD 43,750.00 /each
Part number: ALSU-PSLP-B-HV
Unit Size: 100 Assay Points
List price: USD 660.87
Your price:
USD 660.87
USD 660.87 /each

Overview

Formats:

  • The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
  • The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
  • The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
  • The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.

In the AlphaLISA SureFire Ultra assay, Donor beads are coated with streptavidin to capture one of the antibodies, which is biotinylated. Acceptor beads are coated with a proprietary CaptSure agent that immobilizes the other antibody, labeled with a CaptSure tag. In the presence of target protein, the two antibodies bring the Donor and Acceptor beads close together, generating signal. The amount of light emission is directly proportional to the amount of protein present in the sample.

AlphaLISA SureFire Ultra kits are compatible with:

  • Cell and tissue lysates
  • Antibody modulators
  • Biotherapeutic antibodies

Alpha SureFire kits can be used for:

  • Cellular kinase assays
  • Receptor activation studies
  • Screening

Specifications

Application
Protein Detection
Assay Points
100
Assay Target Class
Phospho-protein
Assay Technology
Alpha
Automation Compatible
Yes
Brand
AlphaLISA SureFire Ultra
Detection Method
Alpha
Experimental Type
In vitro
Therapeutic Area
Autoimmunity
Inflammation
Unit Size
100 Assay Points

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Resources

1-4 of 4 Resources
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Guide
AlphaLISA SureFire Ultra assay optimization

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SDS, COAs, Manuals and more

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