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AlphaLISA SureFire Ultra Human Phospho-SIRPα (pan-Tyr) Detection Kit, Control Lysate
AlphaLISA SureFire Ultra Human Phospho-SIRPα (pan-Tyr) Detection Kit, Control Lysate
Extra control lysate for the AlphaLISA™ SureFire® Ultra™ Human Phospho-SIRPα (pan-Tyr) assay. This control is already provided in the AlphaLISA SureFire Ultra Human Phospho-SIRPα (pan-Tyr) assay kit but can also be ordered separately.
| Feature | Specification |
|---|---|
| Application | Cell Signaling |
| Protocol Time | 2h at RT |
Extra control lysate for the AlphaLISA™ SureFire® Ultra™ Human Phospho-SIRPα (pan-Tyr) assay. This control is already provided in the AlphaLISA SureFire Ultra Human Phospho-SIRPα (pan-Tyr) assay kit but can also be ordered separately.
AlphaLISA SureFire Ultra Human Phospho-SIRPα (pan-Tyr) Detection Kit, Control Lysate
AlphaLISA SureFire Ultra Human Phospho-SIRPα (pan-Tyr) Detection Kit, Control Lysate
Product information
Overview
This control lysate is intended for use with the AlphaLISA SureFire Ultra Human Phospho-SIRPα (pan-Tyr) Detection Kit or for use as a control for those running orthogonal or secondary assays along with the SureFire assay. To prepare the lysate, U937 cells were cultured in 10% FBS containing medium. Cells were harvested, washed with HBSS, adjusted to 106 cells/mL and treated with 500 μM Pervanadate for 15 minutes. Cells were lysed with the addition of 5X Lysis Buffer.
Specifications
| Application |
Cell Signaling
|
|---|---|
| Automation Compatible |
Yes
|
| Brand |
AlphaLISA SureFire Ultra
|
| Detection Modality |
Alpha
|
| Protocol Time |
2h at RT
|
| Shipping Conditions |
Shipped in Blue Ice
|
| Target |
SIRPα
|
| Target Class |
Phosphoproteins
|
| Target Species |
Human
|
| Technology |
Alpha
|
| Therapeutic Area |
Autoimmunity
Oncology
|
| Unit Size |
1 vial
|
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