Skip to main content

AlphaLISA Surefire Ultra Total RSK1 Detection Kit, 50,000 Assay Points

The AlphaLISA® SureFire® Ultra Total RSK1 assay is a sandwich immunoassay for quantitative detection of total RSK1 in cellular lysates using Alpha Technology.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Part Number: ALSU-TRSK1-A500
Unit Size:
Part Number: ALSU-TRSK1-A10K
Unit Size:
Part Number: ALSU-TRSK1-A50K
Unit Size:
Part Number: ALSU-TRSK1-A-HV
Unit Size:
  • The 90 kDa ribosomal S6 kinases (RSK1-4) are a family of widely expressed Ser/Thr kinases characterized by two non-identical, functional kinase domains and a carboxy-terminal docking site for extracellular signal-regulated kinases (ERKs). Several sites, both within and outside the RSK kinase domain, including Ser380, Thr359, Ser363, and Thr573, are essential for kinase activation. RSK1-3 is activated via a coordinated phosphorylation by MAPKs, autophosphorylation, and phosphoinositide-3-OH kinase (PI3K) in response to many growth factors, polypeptide hormones, and neurotransmitters.

    The AlphaLISA® SureFire® Ultra™ Total RSK1 assay is a sandwich immunoassay for quantitative detection of total RSK1 in cellular lysates using Alpha Technology.

    Formats:

    • The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
    • The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
    • The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
    • The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.

    AlphaLISA SureFire Ultra kits are compatible with:

    • Cell and tissue lysates
    • Antibody modulators
    • Biotherapeutic antibodies

    Alpha SureFire kits can be used for:

    • Cellular kinase assays
    • Receptor activation studies
    • Screening
  • Application
    Protein Analysis & Detection
    Protein Detection
    Assay Target Class
    Phospho-protein
    Assay Technology
    Alpha
    Automation Compatible
    Yes
    Brand
    AlphaLISA SureFire Ultra
    Detection Method
    Alpha
    Experimental Type
    In vitro
    Therapeutic Area
    Oncology

Resources

1-1 of 1 Resources
Application Note Icon Image
Application Note
Characterizing Chemokine Receptor Inhibitors with AlphaLISA SureFire Ultra
surefire, neutralizing antibody, neutralizing antibodies, biotherapeutics, biotx