The AlphaLISA™ SureFire® Ultra™ total ribosomal protein S6 (RPS6) assay is a sandwich immunoassay for quantitative detection of Ribosomal S6 (phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be a normalization phosphorylation studies.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).
The AlphaLISA™ SureFire® Ultra™ S6 ribosomal protein (S6RB) assay is a sandwich immunoassay for quantitative detection of total ribosomal protein S6 (RBS6 phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology.
Formats:
In the AlphaLISA SureFire Ultra assay, Donor beads are coated with streptavidin to capture one of the antibodies, which is biotinylated. Acceptor beads are coated with a proprietary CaptSure™ agent that immobilizes the other antibody, labeled with a CaptSure tag. In the presence of target protein, the two antibodies bring the Donor and Acceptor beads close together, generating signal. The amount of light emission is directly proportional to the amount of protein present in the sample.
AlphaLISA SureFire Ultra kits are compatible with:
Alpha SureFire kits can be used for:
Application |
Cell Signaling
|
---|---|
Automation Compatible |
Yes
|
Brand |
AlphaLISA SureFire Ultra
|
Detection Modality |
Alpha
|
Molecular Modification |
Total
|
Product Group |
Kit
|
Sample Volume |
10 µL
|
Shipping Conditions |
Shipped in Blue Ice
|
Target |
Ribosmal Protein S6
|
Target Class |
Phosphoproteins
|
Technology |
Alpha
|
Therapeutic Area |
Inflammation
Metabolic
Oncology
|
Unit Size |
500 Assay Points
|
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