Extra control lysate for the AlphaLISA™ SureFire® Ultra™ Phospho-Ret (pan Tyr) assay. This control is already provided in the AlphaLISA SureFire Ultra Phospho-Ret (pan Tyr) assay kit, but can also be ordered separately.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).
This control lysate is intended for use with the AlphaLISA SureFire Ultra Phospho-Ret (pan Tyr) Detection Kit or for use as a control for those running orthogonal or secondary assays along with the SureFire assay. To prepare the lysate SH-SY5Y cells were cultured to confluence in T175 flasks in 10% FBS containing medium, then treated with 12.5 μM retinoic acid for 16 hours and lysed in 5 mL of 1X SureFire Ultra Lysis Buffer.
Application |
Cell Signaling
|
---|---|
Assay Target |
Ret
|
Assay Target Class |
Phosphoproteins
|
Assay Technology |
Alpha
|
Automation Compatible |
Yes
|
Brand |
AlphaLISA SureFire Ultra
|
Detection Method |
Alpha
|
Molecular Modification |
Phosphorylation
|
Product Group |
Control Lysate
|
Shipping Conditions |
Shipped in Blue Ice
|
Unit Size |
1 Vial
|
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