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AlphaLISA Surefire Ultra Human and Mouse Phospho-Raf-1 (Ser338) Detection Kit, 50,000 Assay Points

The AlphaLISA® SureFire® Ultra™ p-Raf-1 (Ser338) assay is a sandwich immunoassay for quantitative detection of phospho-Raf-1 (phosphorylated on Ser338) in cellular lysates using Alpha Technology.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Part Number: ALSU-PRAF-A500
Unit Size: 500 Assay Point
Part Number: ALSU-PRAF-A10K
Unit Size: 10,000 Assay Points
Part Number: ALSU-PRAF-A50K
Unit Size: 50,000 Assay Points
Part Number: ALSU-PRAF-A-HV
Unit Size: 100 Assay Points
  • The AlphaLISA® SureFire® Ultra™ p-Raf-1 (Ser338) assay is a sandwich immunoassay for quantitative detection of phosphorylated Raf-1 in cellular lysates using Alpha Technology.

    Formats:

    • The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
    • The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
    • The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
    • The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.

    In the AlphaLISA SureFire Ultra assay, Donor beads are coated with streptavidin to capture one of the antibodies, which is biotinylated. Acceptor beads are coated with a proprietary CaptSure™ agent that immobilizes the other antibody, labeled with a CaptSure tag. In the presence of target protein, the two antibodies bring the Donor and Acceptor beads close together, generating signal. The amount of light emission is directly proportional to the amount of protein present in the sample.

    AlphaLISA SureFire Ultra kits are compatible with:

    • Cell and tissue lysates
    • Antibody modulators
    • Biotherapeutic antibodies

    Alpha SureFire kits can be used for:

    • Cellular kinase assays
    • Receptor activation studies
    • Screening
  • Application
    Protein Analysis & Detection
    Assay Points
    50000
    Assay Target Class
    Phospho-protein
    Assay Technology
    Alpha
    Automation Compatible
    Yes
    Brand
    AlphaLISA SureFire Ultra
    Detection Method
    Alpha
    Experimental Type
    In vitro
    Species
    Human
    Therapeutic Area
    Oncology
    Unit Size
    50,000 Assay Points

Resources

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Brochure
Alpha SureFire Ultra no-wash immunoassay catalog

Discover Alpha SureFire® Ultra™ assays, the no-wash cellular kinase assays leveraging Revvity's exclusive bead-based technology...

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