Extra control lysate for the AlphaLISA™ SureFire® Ultra™ Human and Mouse Phospho-Raf-1 (Ser259) assay. This control is already provided in the AlphaLISA SureFire Ultra Human and Mouse Phospho-Raf-1 (Ser259) assay kit but can also be ordered separately.
Feature | Specification |
---|---|
Application | Cell Signaling |
Extra control lysate for the AlphaLISA™ SureFire® Ultra™ Human and Mouse Phospho-Raf-1 (Ser259) assay. This control is already provided in the AlphaLISA SureFire Ultra Human and Mouse Phospho-Raf-1 (Ser259) assay kit but can also be ordered separately.
This control lysate is intended for use with the AlphaLISA SureFire Ultra Human and Mouse Phospho-Raf-1 (Ser259) Detection Kit or for use as a control for those running orthogonal or secondary assays along with the SureFire assay. To prepare the lysate, HeLa cells were cultured to confluence in T175 flasks in 10% FBS containing medium. Cells were treated with 200 ng/mL PMA for 10 minutes then lysed with 4 mL of Lysis Buffer.
Application |
Cell Signaling
|
---|---|
Automation Compatible |
Yes
|
Brand |
AlphaLISA SureFire Ultra
|
Detection Modality |
Alpha
|
Host Species |
Human
Mouse
|
Lysis Buffer Compatibility |
Lysis Buffer
|
Molecular Modification |
Phosphorylation
|
Product Group |
Control Lysate
|
Shipping Conditions |
Shipped in Blue Ice
|
Target |
Raf-1
|
Target Class |
Phosphoproteins
|
Target Species |
Human
Mouse
|
Technology |
Alpha
|
Therapeutic Area |
Oncology
|
Unit Size |
1 vial
|
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