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This control lysate is intended for use with the AlphaLISA SureFire Ultra Human Phospho-RAB29 (Ser72) Detection Kit or for use as a control for those running orthogonal or secondary assays along with the SureFire assay. To prepare the lysate, THP-1 cells were seeded at 500K/mL in fresh medium for 24 hours. Cells were treated with 100 ng/mL Calyculin for 2 hours. Cells were spun down at 300 RCF for 5 minutes, washed with HBSS and lysed with Lysis Buffer at 10x106 cells/mL.
| Application |
Cell Signaling
|
|---|---|
| Automation Compatible |
Yes
|
| Brand |
AlphaLISA SureFire Ultra
|
| Detection Modality |
Alpha
|
| Lysis Buffer Compatibility |
Lysis Buffer
|
| Molecular Modification |
Phosphorylation
|
| Product Group |
Control Lysate
|
| Shipping Conditions |
Shipped in Blue Ice
|
| Target |
RAB29
|
| Target Class |
Phosphoproteins
|
| Target Species |
Human
|
| Technology |
Alpha
|
| Therapeutic Area |
Neuroscience
|
| Unit Size |
1 vial
|
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Dr. Muñoz-Sanjuan, Vice President of Translational Biology at CHDI Foundation, a private non-profit research organization focused...
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